Ansc 107 Part 16- Embryo Transfer

Front 1

embryo transfer

Back 1

the movement of pre-implantation embryos from the repro tract of the genetic mother (donor) to the surrogate mother (recipient)

Front 2

objective of ET

Back 2

to increase # of offspring from genetically valuable females

Front 3

6 steps in the ET process

Back 3

super-ovulation of donor, insemination of donor, synchronization of donor and recipient, collection of embryos from donor, location/grading/storing of embryos, deposition of embryos into recipient's repro tract

Front 4

super ovulation

Back 4

induction of follicular development in excess of the normal number. FSH is the hormone used to induce super ovulation in most species. 4 days of treatments

Front 5

the donor should be bred with __ units of semen __ hours post estrus and __ units of semen __ hours post estrus

Back 5

2 units of semen 12 hours post estrus and 1 unit of semen 24 hours post estrus

Front 6

for best results, estrous cycle of recipient should be within ____ hours of the donor, but acceptable results can be achieved if they are within ____ hours

Back 6

24 hours. 36 hours

Front 7

embryos should be collection ___ days post estrus in the bovine

Back 7

7 days

Front 8

a _____________ is given to prevent rectal contractions by the donor

Back 8

epidural block

Front 9

the _____________ is inserted through the vagina and cervix into the uterus and held in place by an inflatable cuff

Back 9

"Foley catheter"

Front 10

the embryos are flushed out of the donor's uterus using a ____________ that is enriched with calf serum and an antibiotic

Back 10

phosphate buffered saline (PBS) solution

Front 11

the embryos are located using a dissecting microscope and then transferred into a small holding dish where they are graded on __________-

Back 11

quality

Front 12

the technician is careful to place the embryo in the _____________ as ovulation occurred in the donor female

Back 12

same uterine horn (same side of uterus)