Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
24 Cards in this Set
- Front
- Back
Properties used for classification
|
1)virion-shape, size, capsomeric symmetry
2)genome 3)proteins 4)replication 5)physical properties 6)biologic properties |
|
Properties of virion
|
size, shape, capsomeric symmetry
|
|
Properties of genome
|
type of nucleic acid, strandedness, circular or linear, sens, segments, capping, terminal proteins, polyadenylation, nucleotide sequence
|
|
Properties of proteins
|
number, size, function, sequence
|
|
Physical properties
|
pH stability, thermal properties, cation stability, solvent and detergent sensitivity, reationa sensitivity
|
|
Biologic properties
|
serology, host range, pahtogenicity, tissue tropisms, transmission, vectors, geographic distribution
|
|
ICTV Order classification
|
Ends in Virales
example: Monomegalvirales |
|
ICTV Family Classification
|
Ends in Viridae
example:paramyxoviridae, rhabdoviridae, filoviridae (Marburg/Ebola), bornaviridea, |
|
ICTV Subfamily Classification
|
Ends in Virinae
|
|
ICTV Genera and Species Classification
|
Ends in virus
Example: Ebola, Rabies |
|
Dulbecco adapted plaque assay for animal viruses
|
1)mix dilution of phage or virus w/bacteria
2)incubate 3)pour on NA 4)incubate plaques represent lysed cells |
|
Plaque assay used for
|
1)quantify infectious particles - all progeny in plaque have same genome
2)Clone isolate |
|
Latent infections
|
not reproducing currently but may reactivate at any time
Example: HSV and Chicken pox |
|
Fluorencent visualization of center of infection
|
1)tag nucleus
2)tag nucleoli 3)tag viral protein 4)merge image |
|
Hemmagglutination assay
|
multiple carbohydrate receptors on the RBC bind to virus particle which is bound to many other RBC which form a light pink shell in bottom of tube
|
|
Hemadsorption
|
viral proteins bind multiple RBC's allow quantification
|
|
Syncytal plaques
|
lycoproteins of cell membrane cas cell fusion - get giant cell with many nuclei
examples:HSV, HIV, paramyxovirus |
|
PCR
|
used to amplify small genome
|
|
taxonomy
|
Science of classification
|
|
viruses with ss DNA are
|
small and have few genes
|
|
viruses with ds DNA include
|
largest knowns
|
|
Viruses with (-)strand RNA have _______nucleocapsids and some have ________genomes
|
helical
fragmented |
|
Viruses with ds RNA genomes have
|
fragmented genomes and capsids with icosahedral symmetry
|
|
Viruses with reverse transcription step in their replications cycle have ________genomes
|
either DNA or RNA
|