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24 Cards in this Set

  • Front
  • Back
Properties used for classification
1)virion-shape, size, capsomeric symmetry
2)genome
3)proteins
4)replication
5)physical properties
6)biologic properties
Properties of virion
size, shape, capsomeric symmetry
Properties of genome
type of nucleic acid, strandedness, circular or linear, sens, segments, capping, terminal proteins, polyadenylation, nucleotide sequence
Properties of proteins
number, size, function, sequence
Physical properties
pH stability, thermal properties, cation stability, solvent and detergent sensitivity, reationa sensitivity
Biologic properties
serology, host range, pahtogenicity, tissue tropisms, transmission, vectors, geographic distribution
ICTV Order classification
Ends in Virales
example: Monomegalvirales
ICTV Family Classification
Ends in Viridae
example:paramyxoviridae, rhabdoviridae, filoviridae (Marburg/Ebola), bornaviridea,
ICTV Subfamily Classification
Ends in Virinae
ICTV Genera and Species Classification
Ends in virus
Example: Ebola, Rabies
Dulbecco adapted plaque assay for animal viruses
1)mix dilution of phage or virus w/bacteria
2)incubate
3)pour on NA
4)incubate
plaques represent lysed cells
Plaque assay used for
1)quantify infectious particles - all progeny in plaque have same genome
2)Clone isolate
Latent infections
not reproducing currently but may reactivate at any time
Example: HSV and Chicken pox
Fluorencent visualization of center of infection
1)tag nucleus
2)tag nucleoli
3)tag viral protein
4)merge image
Hemmagglutination assay
multiple carbohydrate receptors on the RBC bind to virus particle which is bound to many other RBC which form a light pink shell in bottom of tube
Hemadsorption
viral proteins bind multiple RBC's allow quantification
Syncytal plaques
lycoproteins of cell membrane cas cell fusion - get giant cell with many nuclei
examples:HSV, HIV, paramyxovirus
PCR
used to amplify small genome
taxonomy
Science of classification
viruses with ss DNA are
small and have few genes
viruses with ds DNA include
largest knowns
Viruses with (-)strand RNA have _______nucleocapsids and some have ________genomes
helical
fragmented
Viruses with ds RNA genomes have
fragmented genomes and capsids with icosahedral symmetry
Viruses with reverse transcription step in their replications cycle have ________genomes
either DNA or RNA