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4 Cards in this Set
- Front
- Back
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What is electrophoresis? |
Separation of molecules by using an electrical current based on charge, size and shape Mobility=qE/r q net charge E field strength r molecular radius |
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What is hplc? |
Complicated colum chromatography automated and computer controlled gradient elution using 2 solvent bottles |
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How does reverse hplc work? |
Uses silica gel reacted with hydrocarbon chains to make it non polar then a polar solvent is used instead. This means polar solvents will flow through the column first. |
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What is the significance of retention time? |
It is the time taken for compound to flow through the column to the detector. But there are many variables so conditions must be controlled if this is used as a method of identitifing a compound. The detctor is a UV- visible spectrometer. |
