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22 Cards in this Set
- Front
- Back
These early researchers discovered the nature passive transfer of antibodies, allowing life threatening Diptheria to be treated by serum from immunizided horses
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Von Vehring and Kitasato in 1890
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Which isoform of FcyRII is inhibitory?
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Isoform B is inhibitory
(isoform A is activating) |
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This type of antigen binding molecule is able to accept both linear and conformational determinants
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Antibodies (Ig)
T cell receptors only linear |
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CD marker / Fc receptor found on neutrophils
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FcyRI, high affinity, CD64
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CD marker / Fc receptor found on NK cells
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FcyRIII, low affinity, CD16
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Place, in rank order, serum concentrations of Ig
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G-A-M-E-D
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Which IgG subclass has the shortest halflife?
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IgG3
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Who discovered monoclonal antibodies through use of hybridomas?
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Kohler and Milstein (1975)
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Which CDR region is the most variable?
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CDR3
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Which peptase breaks an antibody into two fragments: Fab (x2) and Fc
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Papain
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Which peptidase breaks an immunoglobulin into an F(ab')2 only?
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Pepsin
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Which Igs contain 3 C regions?
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G - A - D
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Which Igs contain 4 C regions?
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M - E
(Kim said the phrase "I want more... ME = more") |
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Which Ig has the shortest half-life?
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IgE of 2 days (although IgD has half-life of 3 days)
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Which Ig has the longest half-life
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IgG at 23 days
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Which Ig has the largest serum concentration?
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IgG at 13.5 mg/ml
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What are the CD markers for the Iga and IgB of the BCR?
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CD-79a and CD-79b
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What is polyvalency
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Multiple identical determinants in an antigen is referred to as polyvalency or multivalency
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What is done to small immunogens that are not capable of stimulating a humoral response on their own accord? (small chemicals, for example)
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Bind the small chemical, called a hapten, with a protein, called a carrier. This makes the hapten-carrier complex capable of acting like an immunogen
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What are the three major types of determinants?
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Conformational, linear and neoantigenic (the final one caused by proteolysis)
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What is the difference between affinity and avidity?
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Affinity = strength of a single binding site between antibody and epitope
Avidity is strength of entire antibody molecule combining to all epitopes (IgM has higher avidity due to 5 binding sites, for example) |
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What is the only way for an antibody to increase its affinity?
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Affinity maturation increases affinity. No other way.
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