Abac Biol 3500 Lab Midterm Part 1 (Branch)

Front 1

Just after decolorizer is applied acid-fast bacteria will appear ___ and non-acid bacteria will appear ______.

Back 1

Fushcia and colorless

Front 2

After brilliant green is applied as counter stain, acid-fast bacteria will appear _____ while non-acid bacteria appear _____.

Back 2

Fuschia and green

Front 3

Cellular morphology and appearance mycobacterium after the acid-fast stain is ____

Back 3

Fuchsia bacilli

Front 4

Staphylococcus aureus after acid-fast stain is ____

Back 4

Clusters of green cocci

Front 5

E. Coli is Gram ______ (negative/positive)

Back 5

negative

Front 6

Most critical step of gram stain is ___

Back 6

Decolorization

Front 7

If you forgot counter stain at end of stain procedure, gram neg would appear ______ and gram positive would appear _______

Back 7

Colorless

purple

Front 8

Cellular morphology and Gram reaction for Staphylococcus aureus

Back 8

Gram-positive staphylococci

Front 9

Why is it important crystal violet be contrasting color to safranin

Back 9

Difference in color between the two stains allows you to differentiate between gram negative and gram positive cells by observing the color of organism

Front 10

Microscopic morphology of Micrococcus luteus?

Back 10

Gram positive tetrads and staphylococci

Front 11

Purpose of allowing bacteria smear to dry when placed on slide

Back 11

Drying helps remove excess water to ensure optimal heat fix

Front 12

Chains of spherically shaped bacteria are called _____.

Back 12

streptococci

Front 13

Clusters of spherically shaped bacteria are ____

Back 13

Staphylococci

Front 14

Chains of rod-shaped bacteria are ____

Back 14

Streptobacilli

Front 15

Decolorizing agent in spore stain is ____

Back 15

Water

Front 16

Name two genera of bacteria that are acid-fast.

Back 16

Mycobacterium

Nocardia

Front 17

______ bacteria retain carbol fuchsin after acid-alcohol, ______ are decolorized

Back 17

Acid-fast

non-acid fast

Front 18

The bacilli in this stin are gram ____ and the cocci are gram ____

Back 18

Gram negative (pink)
Gram positive (purple)

Front 19

The fushcia cells are acid-fast ____ and the blue-green cells are acid-fast _____.

Back 19

Acid-fast positive (pink)

Acid-fast negative (blue-green)

Front 20

These bacteria are acid-fast _____.

Back 20

Acid-fast negative (fushcia)

Front 21

These bacteria are acid-fast _____.

Back 21

Acid-fast negative (bluish-green)

Front 22

Does this organism have endospores?

Back 22

Endospore positive (green endospores) with pink vegetative cells

Front 23

Does this organism have endospores?

Back 23

Endospore negative

Front 24

1. The genus of this organism is ____.

2. Describe the colonial morphology.

Back 24

1. Serratia (culture media)
2. Red, medium, irregular, circular, umbinate

Front 25

1. The genus of this organism is ____.

2. Describe the colonial morphology.

Back 25

1. Micrococcus (culture media)
2. Bright/buttery yellow, smaller, round, opaque, entire

Front 26

Fomite

Back 26

Object or substance capable of carrying infectious organisms

Front 27

Aseptically

Back 27

Performed under sterile conditions

Front 28

Density terms?

Back 28

Opaque, transparent, or translucent

Front 29

Consistency terms?

Back 29

Butyrous, mucoid, dull, dry, or powdery

Front 30

Texture terms?

Back 30

Smooth, rough, wrinkled, glistening, or irredescent

Front 31

Colony forming unit- CFU

Back 31

Viable bacteria yields one colony

Front 32

TFTC means ___

Back 32

Too few to count

Front 33

TNTC means ___

Back 33

Too numerous to count

Front 34

Define nosocomial infection

Back 34

Infection development favored by a hospital environment

Front 35

List the Gram stain reagents, time, and purpose

Back 35

Crystal violet (primary stain)- 60 sec

Gram's iodine (mordant)-60 sec.

ethyl alcohol (decolorizer) 10-15 sec

safranin (counter stain) 30 sec

Front 36

List the Acid-fast reagents, purpose, and time

Back 36

Carbol fuchsin (primary stain) 5 min

acid-alcohol (decolorizer) 30 sec

brilliant green (counter stain) 2 min

Front 37

List the endospore stain reagents and time

Back 37

Malachite green (endospore stain) -steam 5 min

safranin (vegetative stain)- 1 min

Front 38

1. Describe the microscopic morphology.

2. Name the organism we have seen in lab that could have been used to make this slide.

Back 38

1. Gram positive streptobacilli

2. Bacillus cereus

Front 39

Mycobacterium is acid–fast ___ (positive/negative) and ____ shaped

Back 39

Positive

bacillus

Front 40

1. The genus of this organism is ____.

2. Describe the colonial morphology.

Back 40

1. Bacillus

2. Beige, dry, rough, opaque, large, flat, irregular