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15 Cards in this Set
- Front
- Back
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List the 2 main types of BUN methodologies:
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1. Indirect methods
2. Direct methods |
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List the 3 indirect BUN methods?
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1. Coupled enzymatic
2. Bertholot 3. Conductimetric |
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What is the direct BUN method?
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Diacetyl monoxime
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What is the basic principle of the indirect BUN methods?
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-Urea is converted to CO2 NH4 ion;
-The ammonium ion is then measured. |
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What enzyme is used for "Coupled Enzymatic BUN method"?
What is ultimately measured? |
-Urease enzyme
-Final measurement: NAD |
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What wavelength is NAD measured at?
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340 nm
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What is ultimately measured in the Berthelot method?
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Indophenol Blue product
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Why is the Berthelot method less dependible?
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Ammonia in the air can cause contamination and nonspecific results.
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What is the principle of the Conductimetric method for BUN?
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-Ammonium carbonate is formed after urease treatment
-Conductivity increases |
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What is measured in the Diacetyl monoxime method for BUN?
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a yellow diazine product
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List 3 different methods for testing Creatinine levels:
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1. Jaffe'
2. Jaffe'-Kinetic modification 3. Enzymatic |
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On what reaction are chemical methods for Creatinine determination based?
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Creatinine + picric acid -> orange-red chromagen
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What wavelength is the Jaffe method read at?
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between 490 and 500 nm
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What are interfering substances with the Jaffe method?
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Bilirubin, other nonspecific reactants (glucose/ascorbic acid, etc)
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What reagent can make the Jaffe reaction more specific?
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Lloyd's reagent
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