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25 Cards in this Set
- Front
- Back
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Which enzymes are needed for insertion of a DNA segment?
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Restriction endonuclease-DNA Ligase
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Which receptor does PPAR communicate with?
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RXR
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Which kinase can phosphorylate EPO receptors?
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JAK
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Name the 3 main groups of proteins produced in the liver
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Steroids-Coagulation factors-Albumin
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Function of ghrelin and PPY, and on which organ respectively?
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Ghrelin -> stomach, increase appetite-PPY -> colon/ileum -> lowers appetite
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How are steroid hormones act on lipid storage? |
Steroids bind to HRE - > PP1 CARBOXYLASE > expression of glycerin 3 phosphate decreased |
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Difference btw intracellular and intestinal lipase?
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Intracellular pH7 (ph 4 = lysosome)-Intestinal pH 8
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Function of 14-3-3 protein in FOXO
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Can bind FOXO and keep it in the cytosol
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Function of retinoblastoma protein.
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inhibits cell division -> binds E2F receptor -> tumor suppressor
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Difference between real-time PCR and normal
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In real-time -> quantification is possible
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Difference between transfection and transformation?
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Transfection -> eukaryotes-Transformation -> prokaryotes
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Give two examples of proapoptotic and anti-poptotic proteins
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Pro-apoptotic ->Bad, Bax-Anti-apoptotics -> Bcl2, Bcl-xl
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When is phosphorylation of RNA subunits performed?
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5 ́cap formation-splicing-Poly-A-tail-formation
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Writhe the reaction with abbreviations for fmet-tRNA-synthethase
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tRNA met + 10f-THF -> fMet-tRNA + THF
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How is Ca2+ prostaglandin synthesis activated?
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activated phospholipase A2 -> arachidonic acid -> prostaglandins.
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Write with standard abbreviation the reaction for p450.
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R + O2 + NADPH +H -> H2O + NADP + ROH
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What are the products of phospholipase A2?
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Arachidonic acid-Diacylglycerol (DAG)
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What distinguish the newly created strand from the template strand in the DNA?
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Newly created strand is methylated
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Name 3 proteins that can bind to both ends of the mRNA
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PAB-elF4F-PBR
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What is the difference between hemoglobin A and S?
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Hb-S -> has a point mutation in the 6thpotition (valine instead of glutamate)
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At what stage is the fibroblast growth stopped?2 components
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G1 cyclin-CDK2
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Give the receptor-independent pathway of apoptosis.
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P53 -> caspase 8, 9 -> effectorcaspase 3 -> DNase
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To which family does FAS belong, and what is activated by its domain?
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Family of death receptors-activates -> FADD -> caspase 8
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Give an inhibitor of F0F1 atpase
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Oligomycin
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Give an inhibitor, activator and substrate of cyclin dependent kinase
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Inhibitor: p27-Activator: cyclin-substrate: RbProtein
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