Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
17 Cards in this Set
- Front
- Back
|
Sim medium is used to determine what? |
Sulfur reduction, indole production, & motility |
|
|
What enzymes are present during sulfur reduction? |
Cysteine desulfurase Thiosulfate reductase |
|
|
Cysteine desulfurase is responsible for what? |
Catalyzing sulfur hydrolysis |
|
|
What are the products of sulfur hydrolysis? |
Pyruvate & H2S |
|
|
Thiosulfate reductase is responsible for what? |
Catalyzing sulfur reduction |
|
|
What are the end of products of sulfur reduction? |
H2S |
|
|
What amino acid indicates an indole reaction? |
Tryptophan |
|
|
What enzyme is present in an indole positive organism? |
Tryptophanase |
|
|
What is tryptophanase responsible for? |
Hydrolyzing tryptophan into indole & pyruvate |
|
|
What reagent is added after incubation for the indole production test? |
Kovac’s reagent |
|
|
If the reagent turns red it indicates the presence of what enzyme? |
Tryptophanase |
|
This is a SIM medium testing for sulfur reduction. What is the result & interpretation of the organism on the left? |
No black in the medium. Sulfur is not reduced |
|
This is a SIM medium testing for sulfur reduction? What are the results & interpretation of the organism on the right? |
Black in the medium. Sulfur reduction Motility is impossible to read |
|
These are Indole Production Test Results in SIM Medium. What are the results & interpretation of the organism on the right? |
Red in alcohol layer of Kovac’s reagent, positive. Organism produces tryptophanase & hydrolyzes tryptophan into indole & pyruvate |
|
These are Indole Production Test Results in SIM Medium. What’s the result & interpretation of the organism on the left? |
Reagent color is unchanged, Negative. Tryptophanase is absent & indole is not produced |
|
These SIM tubes were testes for Motility. What is the result & interpretation of the organism on the left? |
No radiating growth, negative. Nonmotile |
|
These SIM tubes were tested for Motility. What are the results & interpretation of the organism on the right? |
Growth radiating outward from stab line, positive. Motility |
