4/3 Corynebacterium Diptheria

Front 1

corynebacterium diphteriae

*related strain C. ulcerans, produces same disease*

Back 1

gram positive club shaped bacilli

facultative

found among normal flora of skin and nasopharynx

grows on Tinsdale Medium
*potassium tellurite, selective as it supresses normal flora, but allows differentiation of C. diptheriae

*Loeffler's coagulated blood serum
non spore forming ROD

Front 2

C Dip DISEASE

'grayish pseudomembrane'

respiratory vs cutaneous

Back 2

RESPIRATORY-
pharyngitis, diptheritic membrane, potential airway obstruction

Cervical lymphadenitis (bull neck)

systemic- fever, fatigue, malaise, *myocarditis*, neuritis

Cutaneous Diptheria-
ulceration, superinfection w/ other bacteria, usually no systemic manifestations

Front 3

C Dip Pathogenesis

Lab DX via culture and showing toxin production

Back 3

colonization of nasopharyngeal mucosa

exotoxin production w/ affects local and system by interfering with protein synthesis

Front 4

Dip Toxin

Back 4

single polypeptide chain cleaved into 2 by furin

1. A = enzymatically active, ADP ribosyl transferase

2. B = binding subunit, binds toxin to cell surface receptor

B subunit binds targets and A enters ...A blocks protein synthesis by inactivating EF2 (by ribosylating it)...damage to heart and neural tissue can be lethal

need lysogenic bacteriophage w/ toxin on genome

Front 5

DIp treatment

(prevent via Toxoid Vaccine)

(vaccination has eliminated respiratory diptheria in US)

Back 5

maintain airway

give anti-toxin

ab = pcn and erythromycin

Front 6

Other Corynebacterium

Back 6

1. C ulcerans - diptheria

2. C jeikeium - sepsis in people w/ cancer, multiple resistance, use vanco

3. Arcanobacterium C. equi - pharyngitis, rash, chronic skin ulcer

4. Rhodococcus C. equi - pulmonary and disseminated infection in HIV patients