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15 Cards in this Set

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Functional component of organs/tissues are called
Parenchyma
Supporting component of organs/tissues are commonly called
Stroma
True or False

In PARAFFIN processing -- fat cells look like great big empty spaces with nuclei due to the alcohol baths
True
What stain is used to cause materials to be BASOPHILIC
Hematoxylin
Name the things that are normally basophilic when stained with HEMATOXYLIN?
Nucleic acids / Ribosomes / Nucleus

calcium deposits

ECM such as GLYCOSAMINOGLYCANS
Describe the Paraffin Process

F.D.I.S.S.
Fixation :: 4% BUFFERED FORMALDEHYDE (10% FORMALIN)

Dehydration and Clearing:: water removed with ALCOHOL 70%-->100% then use CLEARING AGENT (removes NEUTRAL FATS from tissue)

Infiltration and Embedding:: add PARAFFIN

Sectioning:: use MICROTOME

Staining
What stain is used to make materials ACIDOPHILIC or EOSINOPHILIC?
Eosin
What is usually stained with EOSIN?
Membranes and Membranous organelles
-mitochondria
-plasma membranes
-smooth ER

ECM such as COLLAGEN
Stain used in blood smears?
Wright's Stain
Stain used in cytologic smears such as cervix
Papanicolaou Stain

"pap smear"
Stain used that delineates TYPE I COLLAGEN (aqua blue), cytoplasm (red) and nuclei (black)
Trichrome Stain
What does PERIODIC ACID-SCHIFF (PAS) used to stain?
Polysaccharides (pink)

Glycogen, delineates basement membranes and highlights cell walls of fungi
How can you distinguish GLYCOGEN from other PAS-positive material?
DIASTASE

--treat slide with DIASTASE FIRST (removes glycogen)

stain with PAS
--anything stained is therefore something other than glycogen!
Stain used for TYPE III COLLAGEN FIBERS in Reticular CT
Reticulin

(silver or agyrophil stain)
What are Immunohistochemical (immunoperoxidase) stains?
MONOCLONAL Ab directed against specific substances.

Ab are attached with CHROMOGEN (brown color)