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15 Cards in this Set
- Front
- Back
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Functional component of organs/tissues are called
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Parenchyma
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Supporting component of organs/tissues are commonly called
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Stroma
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True or False
In PARAFFIN processing -- fat cells look like great big empty spaces with nuclei due to the alcohol baths |
True
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What stain is used to cause materials to be BASOPHILIC
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Hematoxylin
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Name the things that are normally basophilic when stained with HEMATOXYLIN?
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Nucleic acids / Ribosomes / Nucleus
calcium deposits ECM such as GLYCOSAMINOGLYCANS |
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Describe the Paraffin Process
F.D.I.S.S. |
Fixation :: 4% BUFFERED FORMALDEHYDE (10% FORMALIN)
Dehydration and Clearing:: water removed with ALCOHOL 70%-->100% then use CLEARING AGENT (removes NEUTRAL FATS from tissue) Infiltration and Embedding:: add PARAFFIN Sectioning:: use MICROTOME Staining |
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What stain is used to make materials ACIDOPHILIC or EOSINOPHILIC?
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Eosin
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What is usually stained with EOSIN?
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Membranes and Membranous organelles
-mitochondria -plasma membranes -smooth ER ECM such as COLLAGEN |
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Stain used in blood smears?
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Wright's Stain
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Stain used in cytologic smears such as cervix
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Papanicolaou Stain
"pap smear" |
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Stain used that delineates TYPE I COLLAGEN (aqua blue), cytoplasm (red) and nuclei (black)
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Trichrome Stain
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What does PERIODIC ACID-SCHIFF (PAS) used to stain?
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Polysaccharides (pink)
Glycogen, delineates basement membranes and highlights cell walls of fungi |
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How can you distinguish GLYCOGEN from other PAS-positive material?
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DIASTASE
--treat slide with DIASTASE FIRST (removes glycogen) stain with PAS --anything stained is therefore something other than glycogen! |
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Stain used for TYPE III COLLAGEN FIBERS in Reticular CT
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Reticulin
(silver or agyrophil stain) |
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What are Immunohistochemical (immunoperoxidase) stains?
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MONOCLONAL Ab directed against specific substances.
Ab are attached with CHROMOGEN (brown color) |
