Scientists have the ability to determine the molarity and the contents of a substance by measuring the intensity of the light that is emitted through the solution. Scientists accomplish this by using the technique of spectroscopy; spectroscopy can be defined as the science affiliated with the spectra produced when matter interacts with light. Lab 7 investigation 19, “What Factors Affect the Intensity of Color?” expands on the process of spectroscopy, and its relation to the molarity of a substance. The goal of this lab was to determine the effects of the intensity of color of a solution (2). Spectroscopy is frequently used in the medical field by using the process of infrared spectroscopy, which is used to examine biological fluids. This can
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In real life, spectroscopy is often used in the medical field to examine bodily fluids through the process of infrared spectroscopy (IR spectroscopy). This technique of spectronomy can identify the chemical makeup of tissue and help doctors spot discrepancies from normal, healthy tissue. “Transmission of light at a given wavelength through tissue depends on a combination of reflectance, scattering, and absorptive effects” (1). The reflectance, scattering, and absorptive effects all complicate the IR spectroscopy process and resulted. Both processes used in real life and the lab have several sources of error. For the lab, a main source of error was due to the colorimeter using wavelengths that varied from the set wavelength indicated on the device. For the real life medical application, one of the errors in IR spectroscopy is due to non metabolized tissue, which is saturated with oxygen. This creates a very large range of results (1). One of the major issues with IR spectroscopy is due to variance within the patients examined. These variations between patients becomes visible in the patient’s arterial/venous ratios when the cerebral is examined. This complications the technique and adds another source of error when examining and diagnosing patients (1). The lab used a similar technique when examining the colored solutions (2). Similar errors can occur due to the water and particles in the solutions tested, much like the saturated tissue disrupts the IR spectroscopy
In real life, spectroscopy is often used in the medical field to examine bodily fluids through the process of infrared spectroscopy (IR spectroscopy). This technique of spectronomy can identify the chemical makeup of tissue and help doctors spot discrepancies from normal, healthy tissue. “Transmission of light at a given wavelength through tissue depends on a combination of reflectance, scattering, and absorptive effects” (1). The reflectance, scattering, and absorptive effects all complicate the IR spectroscopy process and resulted. Both processes used in real life and the lab have several sources of error. For the lab, a main source of error was due to the colorimeter using wavelengths that varied from the set wavelength indicated on the device. For the real life medical application, one of the errors in IR spectroscopy is due to non metabolized tissue, which is saturated with oxygen. This creates a very large range of results (1). One of the major issues with IR spectroscopy is due to variance within the patients examined. These variations between patients becomes visible in the patient’s arterial/venous ratios when the cerebral is examined. This complications the technique and adds another source of error when examining and diagnosing patients (1). The lab used a similar technique when examining the colored solutions (2). Similar errors can occur due to the water and particles in the solutions tested, much like the saturated tissue disrupts the IR spectroscopy