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13. What is the process resulting in antigen and antibody moving toward each other?
One dimensional diffusion
Enzyme-Linked Immunosorbent Assay
14. In the “sandwich” analogy of the direct ELISA, what is the “bread”?
Antibodies
What is the filling? Antigens
15. Describe some advantages of using the ELISA technique to process a large number of samples. The advantage of Sandwich ELISA is that the sample does not have to be purified before analysis, and the assay can be very sensitive.
16. Which patient(s) were positive for HIV?
Patient C is HIV positive
17. Describe what would happen if you skipped the step where the developing buffer was added.
The developing buffer contains the secondary antibodies that the enzyme is conjugated with and without that it wouldn’t develop right and the results would be inconclusive or “retest”
Western Blotting Technique
18. The Western blot technique is used as the confirmatory test for a positive ELISA result because it provides better specificity. How does this technique provide better specificity?
The Western blot provides better specificity because it detects antibodies binding
One dimensional diffusion
Enzyme-Linked Immunosorbent Assay
14. In the “sandwich” analogy of the direct ELISA, what is the “bread”?
Antibodies
What is the filling? Antigens
15. Describe some advantages of using the ELISA technique to process a large number of samples. The advantage of Sandwich ELISA is that the sample does not have to be purified before analysis, and the assay can be very sensitive.
16. Which patient(s) were positive for HIV?
Patient C is HIV positive
17. Describe what would happen if you skipped the step where the developing buffer was added.
The developing buffer contains the secondary antibodies that the enzyme is conjugated with and without that it wouldn’t develop right and the results would be inconclusive or “retest”
Western Blotting Technique
18. The Western blot technique is used as the confirmatory test for a positive ELISA result because it provides better specificity. How does this technique provide better specificity?
The Western blot provides better specificity because it detects antibodies binding