The chromatography was performed on an ACQUITY TM UPLC system (Waters Corp., Milford, MA, USA). The UPLC system included quaternary solvent manager, a binary pump, degasser, autosampler with an injection loop of 10 μL and a column heater-cooler. The separation was performed on Acquity UPLC BEH TM C18 column (50 × 2.1 mm, i.d., 1.7 μm, Waters, USA) maintained at 40 °C. a mobile phase of acetonitrile containing 0.1% formic acid and aqueous 5 mM ammonium acetate buffer containing 0.1% formic acid (80:20, v/v) at a flow rate 0.5ml/min. RIS,9-OH-RIS and IS was eluted at 1.01,0.95 and 1.04 min respectively with a total run time of 2 min only. The injection volume was 5 μL in partial loop mode and the temperature of the autosampler was kept at 15 °C.
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The ESI source was operated in positive ionization mode. Quantification was performed using multiple reaction monitoring (MRM) of the transitions of m/z z 411.3>191.12 ,427>206.97 and 313.0>256.03were used to quantify risperidone ,9-OH-risperidone and IS respectively, with the dwell time of 0.161 s. Nitrogen was used as a desolvating gas at a flow rate of 600 L/h. The desolvating temperature was 350 °C whereas source temperature was 150 °C. The collision gas (argon) flow was 0.1 mL/min and capillary voltage was set at 4
The ESI source was operated in positive ionization mode. Quantification was performed using multiple reaction monitoring (MRM) of the transitions of m/z z 411.3>191.12 ,427>206.97 and 313.0>256.03were used to quantify risperidone ,9-OH-risperidone and IS respectively, with the dwell time of 0.161 s. Nitrogen was used as a desolvating gas at a flow rate of 600 L/h. The desolvating temperature was 350 °C whereas source temperature was 150 °C. The collision gas (argon) flow was 0.1 mL/min and capillary voltage was set at 4