Tissue Physiology Lab Report

Improved Essays
Almost all multicellular organisms contain a nervous system, which allows them to sense changes in their environment or induce muscle contractions through electrical impulses. The majority of nerves cells that make up the nervous system are excitable cells, as they have the ability to produce an electric current to formrom an action potential from using a concentration gradient of ions across their membrane (Sherwood, 2013). The current generated by the flow of sodium (〖Na〗^+) and potassium ( K^+)ions is called an action potential and occurs in three steps: depolarization, repolarization, and hyperpolarization. First, a membrane potential 〖(V〗_m) is established by creating a concentration gradient of 〖Na〗^+ and K^+where there is a high concentration …show more content…
Initially, the sodium potassium pump creates the -70 mV V_m by pumping 〖Na〗^+ out of the cell and K^+ into the cell. After a stimulus is received, the 〖Na〗^+ ion channels open, allowing 〖Na〗^+ to flow into the cell and causing depolarization. Next, repolarization occurs as K^+ ion channels begin to open and K^+ ions flow out of the cell while the 〖Na〗^+ channels begin to close, which . eEstablishinges this a new V_m concentration of K^+ outside the cell and 〖Na〗^+ inside the cell is called repolarization. After repolarization, a new concentration gradient has been established where the K^+ concentration is highest outside the cell and the 〖Na〗^+concentration is highest inside the cell. The third step, hyperpolarization, occurs because of the K^+ channel’s slow response rate in comparison to that of the 〖Na〗^+ channels. During this period another action potential cannot be produced because the V_m has not been fully restored and more importantly because the potassium channels cannot be reopened immediately. The time interval before another action potential can be produced is called the refractory period (Sherwood,

Related Documents

  • Improved Essays

    Ventricles Case Study

    • 734 Words
    • 3 Pages

    • P wave – electrical changes of atrial depolarization. • QRS complex – electrical associated with ventricular depolarization. • T wave – electrical associated with ventricular repolarization. • P-Q segment – associated atria plateau. (no electrical charge) • S-T segment – associated with ventricles plateau.…

    • 734 Words
    • 3 Pages
    Improved Essays
  • Superior Essays

    Purpose and Background Cells divide in two ways, mitosis and meiosis. Mitosis is used to produce cells that are genetically identical to the parent cell for growth, asexual reproduction, or repair after injury. Cells that are produced by mitosis are diploid, meaning that they have two complete sets of chromosomes, one from each parent. Meiosis is used to produce haploid cells that have only one set of chromosomes, a mix of chromosomes from both parents. Meiosis produces cells that are genetically unique from their parent cells.…

    • 1551 Words
    • 7 Pages
    Superior Essays
  • Improved Essays

    Interactive Question 7.2 Cite some experimental evidence that indicates that membrane proteins drift. A good form of experimental evidence is Fluorescence Recovery After Photobleaching, aka FRAP. In the FRAP process, membrane proteins are labelled with a green fluorescent protein, and part of the plasma membrane is “bleached” with a laser, causing them to lose their fluorescence. The part that was bleached will eventually become fluorescent again, as molecules drift in by diffusion.…

    • 853 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Biology Unit 4 Lab Report

    • 543 Words
    • 3 Pages

    Biology 15 Lab # 4 Professor Passerini September 23, 2015 Scot Albert Lab #4 Questions 1a,b,c, 2, 3a,b,c, 4, 5a,b,c, 7, 11c, d, e, 12a,b ---------------------------------------------------------- 1- a-They are found primarily in the thylakoid membranes. b-No. Cyanobacteria do not have distinct nuclei.…

    • 543 Words
    • 3 Pages
    Improved Essays
  • Great Essays

    Myelin Case Study

    • 1830 Words
    • 8 Pages

    An axon that is surrounded by myelin sheath, whether in the central nervous system (CNS) or the peripheral nervous system (PNS), is said to be “myelinated”, while the bare axon is “unmyelinated”. Action potentials travel more rapidly and further in distance along a myelinated axon, this is because they travel by salutatory conduction. This is when the impulse at the first node generates currents that open gated channels…

    • 1830 Words
    • 8 Pages
    Great Essays
  • Improved Essays

    Muscles Lab Report

    • 786 Words
    • 4 Pages

    However, there are a great deal of muscles and nerves in the forearm. When a nerve impulse transmission to synaptosomes induced depolarization, allows calcium ions to enter the cell membrane, the synaptic vesicles move forward and release acetylcholine (ACH). ACH combined with membrane receptors on the endplate cause electronic potentials, potassium and sodium ions exchanging start to spread both sides of muscle cell membrane to form the action potentials, and long with the cell membrane so that both sides of the terminal cistern will release Ca2+, Ca2+ and troponin binding to change tropomyosin, let actin exposed binding site to the cross bridge.…

    • 786 Words
    • 4 Pages
    Improved Essays
  • Superior Essays

    Once the cell begins to hyperpolarize, sodium channels open up and allow sodium ions to flood into the membrane. Once the concentration of sodium ions have been let in, the sodium channel is inactivated. Once the inactivation gate is closed, potassium channels open up so the cell can depolarize. This action allows the cell to restore itself to resting membrane potential. The sodium channels then become closed, and the process is restarted and an action potential has been fully propagated.…

    • 1100 Words
    • 4 Pages
    Superior Essays
  • Improved Essays

    Glt1 Lab Report

    • 847 Words
    • 4 Pages

    SGLT1 uses the sodium gradient to move the glucose molecule down the gradient since it does not have its own energy to do so [6]. The sodium ion pulls molecule with it as it goes back into the cell through carrier proteins. Na+/K+ Pump and the Heart The regulation of Na+/K+-ATPase is important to the muscle tissue of the heart, the myocardial [4]. The ATPase enzymes determine the “set point” and regulate contraction of the cardiac and vascular muscles [4].…

    • 847 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Some studies theorized that ongoing muscle contractions can cause the sodium-potassium pump to temporary increase helping the muscle cells membrane to be more hyperpolarized (Colding-Jorgensen 2005). Hyperpolarization assists with accelerating muscle relaxation (Colding-Jorgensen 2005). In addition to physical…

    • 687 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Skeletal Muscle System

    • 413 Words
    • 2 Pages

    and then travels to the end of the motor neuron. The motor end plate is protected by a phospholipid bilayer that has acetylcholine receptors that allow acetylcholine to flow from the synaptic cleft into the motor end plate. When acetylcholine attaches to the acetylcholine receptors, it activates sodium channels to open. Sodium channels open and let positively charged sodium ions rapidly into the motor end plate, while potassium ions are slowly kicked out of the charged environment in the motor end plate. This is the first step of skeletal muscle fiber excitation, where our nervous system sends a nerve signal to our muscle fibers to go from a relaxed position to a contracted position by producing tension on the skeletal muscle of an area to cause the body to move.…

    • 413 Words
    • 2 Pages
    Improved Essays
  • Decent Essays

    When the neuron is in a resting state with -70mV, there is a great amount of potassium ions inside the cell, and a plethora of sodium ions outside the cell. For something to initiate an action potential, there must be a certain stimulus or impulse. This stimulus would cause voltage-gated sodium channels to open. As sodium flows into the neuron, it must…

    • 429 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    Acute Cocaine Experiment

    • 746 Words
    • 3 Pages

    Type II neurons had medium cell bodies, stellate shaped, five primary dendrites, and did not exhibit thinning varicosities. Hyperpolarizing steps were done and they found that the subsequent Ih current sag, as well as leak channel function, was larger in the type II neurons in comparison to the type I neurons. When looking at intrinsic excitability, type I neurons spiked more often than the type II neurons over the depolarizing steps. A slow wave oscillation firing was also observed in only the type II neurons. These measures allowed for the conclusion that type I and type II cells in the VTA can be categorized based on electrophysiological…

    • 746 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    The overall amplitude of action potential decreased as the concentration ratio of 〖Na〗^+ decreased. Lowest amplitude was observed when the 〖Na〗^+ concentration ratio was set at 0.1 and the highest amplitude was observed when the 〖Na〗^+ concentration ratio was set at 1. The depolarization and repolarization slopes along with g_Na and g_K decreased as the 〖Na〗^+ concentration ratio approached 0mV (Figure 4). Figure five shows how altering E_K through ion substitution changes g_x of ions involved in an action potential. The depolarization slope of the action potential remained unaffected by the change in the K^+ concentration ratio.…

    • 401 Words
    • 2 Pages
    Improved Essays
  • Improved Essays

    Electrophysiology involves using electric conductance to measure the electrical activity of cells and tissues in biological samples. It regulates the electrical activity through a change in voltage or electric current. Specifically, in neuronal cells, it helps in the assessment of action potential, long-term potentiation (LTP) and long term depression (LTD)-all of which play an important role in the synaptic activity of the cell. Patch Clamp technique is one of the widely used electrophysiology studies in neuroscience research. It incorporates the use of a microelectrode with a micropipette that allows drawing a small piece of the cell membrane to study the activity of ion channels.…

    • 559 Words
    • 3 Pages
    Improved Essays
  • Superior Essays

    Positively charged ions are pumped through the membrane creating a slightly more negative atmosphere inside the cell then the outside. This action causes the cell membrane to be polarized. Positively charged ions are kept out and only make it back in through special membrane gate channels which can open and close and these channels are distributed along the axons and dendrites. Changes in the environment stimulate the normally closed channels on the axon to swing open and allow positive charges to rush in through the action of depolarization. This activity causes the next area of the axon to become depolarized resulting in the opening of the neighboring gate.…

    • 2033 Words
    • 9 Pages
    Superior Essays