Irreversible brain damage can be the result of thyroid hormone deficiency even if the deficiency is for short period of time. Thyroid hormone deficiency consequences does not depend on severity itself however, it is also based on the particular timing of onset and duration time period. The mice have been the main target in the study of thyroid physiology and the thyroid hormone actions in the brain. In order to do so, two types of mice have been used: knock out (KO) and knock in (KI) thus expanding our understanding of the thyroid. In KO type, the mice are genetically engineered in order to prevent the expression of specific proteins whereas in KI type, the mice have been injected a specific gene into the target genome by using the homologous recombination. …show more content…
The first antibody is not visible under the microscope since it is unlabelled and therefore, secondary antibody is used which has been popular since 1970s. The secondary antibody is usually labelled in some way to make it visible or detectable. We will be using EnVisionTM FLEX secondary antibody diluent which is recommended for the dilution of Dako concentrated Primary Antibodies. The primary antibody has been grown in either mouse (mouse IgG) or rabbit (rabbit IgG) whereas; the secondary antibody has both anti-mouse and anti-rabbit antibodies on it. The MCT8 primary antibody was used in two different concentrations: 1:200 and 1:500 using the same