Identification of Analgesic Drugs by Thin-Layer Chromatography
1
(a)
In thin-layer chromatography alumina or silica is used as the adsorbent material.
(b)
The filter paper is placed inside the beaker during separation to act as an adsorbent medium.
(c)
The ultraviolet lamp is used after TLC separation to illuminate the TLC sheet.
(d)
Aspirin, acetaminophen, and caffeine are components of analgesic drugs examined in this experiment.
2
A 6cm by 10cm pre-coated TLC sheet will be obtained. On the powdery surface of the TLC sheet, a light pencil line 1cm from the TLC sheet will be drawn. On this light pencil mark, five small dots will be drawn, 1cm between the dots and 1cm from the edges of TLC sheet. On the top of each drawn dot, will be marked as A (aspirin), Ac (acetaminophen), C (caffeine), M (mixtures of the three analgesic drug components) and U (unknown analgesic drug). The fine capillary tube will be used to apply analgesic drug components on the dots. For instance, the fine capillary tube will be dipped in a stock aspirin solution, followed by touching the tip of the capillary tube and quickly transferring the solution to the dot marked A. The solvent at dot marked A will be allowed to evaporate. After evaporation of the solvent, another drop will be added to the same spot. This treatment will be conducted to other dots, but using separate fine capillary tubes and different stock solutions of analgesic drug components. 9cm filter paper will be placed against the wall of …show more content…
Ethyl acetate measurement should be performed in a hood chamber to prevent inhalation. On the other hand, methanol is flammable and should be handled with care. The solvents in this experiment should not be allowed to come in contact with the skin. However, if by accident they come in contact with the skin, the area of contact should be rinsed thoroughly with cold water and
1
(a)
In thin-layer chromatography alumina or silica is used as the adsorbent material.
(b)
The filter paper is placed inside the beaker during separation to act as an adsorbent medium.
(c)
The ultraviolet lamp is used after TLC separation to illuminate the TLC sheet.
(d)
Aspirin, acetaminophen, and caffeine are components of analgesic drugs examined in this experiment.
2
A 6cm by 10cm pre-coated TLC sheet will be obtained. On the powdery surface of the TLC sheet, a light pencil line 1cm from the TLC sheet will be drawn. On this light pencil mark, five small dots will be drawn, 1cm between the dots and 1cm from the edges of TLC sheet. On the top of each drawn dot, will be marked as A (aspirin), Ac (acetaminophen), C (caffeine), M (mixtures of the three analgesic drug components) and U (unknown analgesic drug). The fine capillary tube will be used to apply analgesic drug components on the dots. For instance, the fine capillary tube will be dipped in a stock aspirin solution, followed by touching the tip of the capillary tube and quickly transferring the solution to the dot marked A. The solvent at dot marked A will be allowed to evaporate. After evaporation of the solvent, another drop will be added to the same spot. This treatment will be conducted to other dots, but using separate fine capillary tubes and different stock solutions of analgesic drug components. 9cm filter paper will be placed against the wall of …show more content…
Ethyl acetate measurement should be performed in a hood chamber to prevent inhalation. On the other hand, methanol is flammable and should be handled with care. The solvents in this experiment should not be allowed to come in contact with the skin. However, if by accident they come in contact with the skin, the area of contact should be rinsed thoroughly with cold water and