Thiamine hydrochloride is structurally similar to thiamine pyrophosphate (TPP). TPP is a coenzyme universally present in all living systems. It catalyzes several biochemical reactions in natural systems. It was originally discovered as a required nutritional factor (vitamin) in humans by its link with the disease beriberi. Beriberi is a disease of peripheral nervous system caused by a deficiency of Vitamin B in the diet. Symptoms include pain and paralysis of extremities, emaciation, and swelling of the body. The disease is most common in Asia.
3.2) Theory and research behind
Thiamine binds to an enzyme before the enzyme is activated. The enzyme also binds to the substrate (a large protein). Without the coenzyme thiamine, no chemical reaction would occur. The coenzyme is a chemical reagent. The protein molecule (The enzyme) helps and meditates the reaction by controlling astrochemical, energetic, and entropic factors, but it is nonessential to the overall course of reaction that it catalyzes. A special name, vitamins, is given to coenzymes that are essential to the nutrition of the organism.
4. Procedure:
4.1) Procedure …show more content…
Students first added 0.30 g thiamine hydrochloride to a 25-mL Erlenmeyer flask. Dissolved the solid in 0.45 mL of water by swirling the flask. Add 3.0 mL of 95% ethanol and swirl the solution until it is homogeneous. To this solution it was added 0.90 mL of an aqueous sodium hydroxide solution and then it was swirled in the flask until the bright yellow color faded to a pale yellow. Students then swirled the contents of the flask until it was homogeneous. Stopped the flask and was let to stand in a dark place for 2 days (which was recommended as
3.2) Theory and research behind
Thiamine binds to an enzyme before the enzyme is activated. The enzyme also binds to the substrate (a large protein). Without the coenzyme thiamine, no chemical reaction would occur. The coenzyme is a chemical reagent. The protein molecule (The enzyme) helps and meditates the reaction by controlling astrochemical, energetic, and entropic factors, but it is nonessential to the overall course of reaction that it catalyzes. A special name, vitamins, is given to coenzymes that are essential to the nutrition of the organism.
4. Procedure:
4.1) Procedure …show more content…
Students first added 0.30 g thiamine hydrochloride to a 25-mL Erlenmeyer flask. Dissolved the solid in 0.45 mL of water by swirling the flask. Add 3.0 mL of 95% ethanol and swirl the solution until it is homogeneous. To this solution it was added 0.90 mL of an aqueous sodium hydroxide solution and then it was swirled in the flask until the bright yellow color faded to a pale yellow. Students then swirled the contents of the flask until it was homogeneous. Stopped the flask and was let to stand in a dark place for 2 days (which was recommended as