The Effect of Cellular Respiration at Low and High Temperatures
Introduction
Cellular respiration can include two processes, aerobic respiration and anaerobic respiration. The main purpose of cellular respiration is to breakdown sugars into smaller structures so that the cell can use it as energy (Reece, et al. 2014). The main concept of the respiration lab is aerobic respiration. This is the process that organic compounds will be degraded from oxygen to produce water, carbon dioxides, and different forms of energy such as ATP (Reece, et al. 2014). Cellular respiration is important to life because breaking down sugars into usable energy enables living organisms to function to support their life. This topic relates to our lab experiment because …show more content…
Not only the temperature but the cell type and size, and also how much nutrients is available. The organism that we studied was germinating mung bean seeds. We used a system called respirometer which includes a test tube that is to be filled with the seeds until approximately 3 cm before the top. Other parts that were included was the syringe, and a 1ml pipette, with both the syringe and the pipette threaded through a cork cap that is placed onto the test tube. We are trying to measure the respiration rate. We achieved this by using the respirometer with the syringe fully depressed. Since KOH is used as a carbon dioxide absorbent, we know that the carbon dioxide is introduced. This will cause the KOH to move down since the offset in pressure between the external environment and the internal of the test tube. The alternate hypothesis for this experiment is that if cellular respiration is occurring within the test tube, then the potassium hydroxide level will drop within the pipette because it is absorbing the carbon dioxide. The temperature has the effect to slow down …show more content…
The moment the KOH hits 0ml that is when you start timing. With increments of 5minutes on both the control and the mung bean respirometer, collect the data of how much the KOH increased or decreased. This process is repeated until the 40-minute mark or until the KOH completely drops down.
Completion of Experiment
Upon completion of the experiment, or when the last data has been noted down, the next instructions are to safely remove the cork cap without the KOH spilling onto you. The method to do this task is to slowly push down on the syringe, and have your finger blocking the airflow of the pipet. This stabilizes the KOH within the pipet. Rinse the pipet and the cork cap in the sink and proceed to the next step.
Collecting Stats of the Experiment
Following up after rinsing the pipet and the cork cap of the respirometer, the next step is to weigh the mung bean seeds on a scale to the nearest 0.1g. Note the weight of it and proceed to rinsing only the test tube with the mung bean
Introduction
Cellular respiration can include two processes, aerobic respiration and anaerobic respiration. The main purpose of cellular respiration is to breakdown sugars into smaller structures so that the cell can use it as energy (Reece, et al. 2014). The main concept of the respiration lab is aerobic respiration. This is the process that organic compounds will be degraded from oxygen to produce water, carbon dioxides, and different forms of energy such as ATP (Reece, et al. 2014). Cellular respiration is important to life because breaking down sugars into usable energy enables living organisms to function to support their life. This topic relates to our lab experiment because …show more content…
Not only the temperature but the cell type and size, and also how much nutrients is available. The organism that we studied was germinating mung bean seeds. We used a system called respirometer which includes a test tube that is to be filled with the seeds until approximately 3 cm before the top. Other parts that were included was the syringe, and a 1ml pipette, with both the syringe and the pipette threaded through a cork cap that is placed onto the test tube. We are trying to measure the respiration rate. We achieved this by using the respirometer with the syringe fully depressed. Since KOH is used as a carbon dioxide absorbent, we know that the carbon dioxide is introduced. This will cause the KOH to move down since the offset in pressure between the external environment and the internal of the test tube. The alternate hypothesis for this experiment is that if cellular respiration is occurring within the test tube, then the potassium hydroxide level will drop within the pipette because it is absorbing the carbon dioxide. The temperature has the effect to slow down …show more content…
The moment the KOH hits 0ml that is when you start timing. With increments of 5minutes on both the control and the mung bean respirometer, collect the data of how much the KOH increased or decreased. This process is repeated until the 40-minute mark or until the KOH completely drops down.
Completion of Experiment
Upon completion of the experiment, or when the last data has been noted down, the next instructions are to safely remove the cork cap without the KOH spilling onto you. The method to do this task is to slowly push down on the syringe, and have your finger blocking the airflow of the pipet. This stabilizes the KOH within the pipet. Rinse the pipet and the cork cap in the sink and proceed to the next step.
Collecting Stats of the Experiment
Following up after rinsing the pipet and the cork cap of the respirometer, the next step is to weigh the mung bean seeds on a scale to the nearest 0.1g. Note the weight of it and proceed to rinsing only the test tube with the mung bean