The density and viability results from the trypan blue staining test, shown in table 1 and graph 1, were calculated using formula 1 and formula 2. The results from graph 1 and table 1 show that the untreated cells have the best viability, at 97.5%. Additionally, the results from the differential fluorescence staining and the gelatin zymography are limited because the cells treated with 10% vitamin D3 and the cells treated with 30% vitamin D3 and retinoic acid both had low viability, with 78.6% and 70% respectively. The cells treated with 10% vitamin D3 and the cells treated with 30% vitamin D3 and retinoic acid had low viability because of the stressed placed on the cells when they were treated with the chemical inducers. When HL-60 cells are
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The trypan blue and differential fluorescence staining showed that the 30% vitamin D3 and retinoic acid had a higher density of differentiated cells and the gelatin zymography showed that those differentiated cells were monocytes which supported the hypothesis. Since the results have proved that the synergistic effect would be an effective monocytic chemical inducer of HL-60 cells, this method could be using in real life. These results are very important for the advancement of the differentiation therapy because now doctors will have an option to produce monocytic differentiation in their patients with bad allergies, by using the synergistic effect of vitamin D3 and retinoic acid, instead of using just retinoic acid to produce granulocytic differentiation, which is linked to allergic reactions (9). This will make the recovery process for patients with bad allergies more …show more content…
Some researchers are looking into different chemical inducers that could induce cancer stem cell differentiation in the early stages to prevent the formation of tumors (10). Takahashi, Yamanaka, Jaenisch and Young recently had a breakthrough in which they induced an adult somatic cell to dedifferentiate into pluripotent stem cells by forcing the expression of certain transcription factors, more specifically Oct4, Sox2, Klf4, and c-Myc. Further research can also be in the area of dedifferentiation of adult cancer cells back into stem cells that can then be differentiated into useful safe cells
The trypan blue and differential fluorescence staining showed that the 30% vitamin D3 and retinoic acid had a higher density of differentiated cells and the gelatin zymography showed that those differentiated cells were monocytes which supported the hypothesis. Since the results have proved that the synergistic effect would be an effective monocytic chemical inducer of HL-60 cells, this method could be using in real life. These results are very important for the advancement of the differentiation therapy because now doctors will have an option to produce monocytic differentiation in their patients with bad allergies, by using the synergistic effect of vitamin D3 and retinoic acid, instead of using just retinoic acid to produce granulocytic differentiation, which is linked to allergic reactions (9). This will make the recovery process for patients with bad allergies more …show more content…
Some researchers are looking into different chemical inducers that could induce cancer stem cell differentiation in the early stages to prevent the formation of tumors (10). Takahashi, Yamanaka, Jaenisch and Young recently had a breakthrough in which they induced an adult somatic cell to dedifferentiate into pluripotent stem cells by forcing the expression of certain transcription factors, more specifically Oct4, Sox2, Klf4, and c-Myc. Further research can also be in the area of dedifferentiation of adult cancer cells back into stem cells that can then be differentiated into useful safe cells