The Effect of Dri-Contrad on Phagocytosis in Tetrahymena
Methods:
A control group of Tetrahymena was first made up of 450 microliters of Tetrahymena and 450 microliters of carbon solution in a microcentrifuge tube and a timer was immediately started. 100 microliters of iodine were added to three other microcentrifuge tubes which were labeled C3, C6, and C9 to act as a stop solution by killing the Tetrahymena. At three minutes, 100 microliters of solution were pipetted from the control tube to the tube labeled C3. This process was repeated at 6 and 9 minutes. A wet mount was then made of 50 microliters from each C3, C6, and C9 tube to their corresponding glass slide. The slides were observed one by one under a dissecting microscope. The number of food vacuoles seen in the first 10 Tetrahymena cells were recorded for each slide. After the 10 Tetrahymena were found on each slide the slides were placed into a discard bin in the center of the table. The food vacuoles found on each slide were then averaged to get the mean number at each time point which was recorded in a separate table. …show more content…
200 microliters of the Dri-Contrad stock chemical, 200 microliters of the carbon solution, and 200 microliters of Tetrihymena were all added to a microcentrifuge tube labeled X-U. After 3, 6, and 9 minutes, 100 microliters of solution from the X-U tube was transferred to three tubes labeled 3X-U, 6X-U, and 9X-U. A wet mount was made of 50 microliters from each tube to their corresponding glass slide. Each slide was then observed under a dissecting microscope and the number of food vacuoles seen in the first 10 Tetrahymena cells were recorded and averaged to get the mean number of food vacuoles per cell at each time
Methods:
A control group of Tetrahymena was first made up of 450 microliters of Tetrahymena and 450 microliters of carbon solution in a microcentrifuge tube and a timer was immediately started. 100 microliters of iodine were added to three other microcentrifuge tubes which were labeled C3, C6, and C9 to act as a stop solution by killing the Tetrahymena. At three minutes, 100 microliters of solution were pipetted from the control tube to the tube labeled C3. This process was repeated at 6 and 9 minutes. A wet mount was then made of 50 microliters from each C3, C6, and C9 tube to their corresponding glass slide. The slides were observed one by one under a dissecting microscope. The number of food vacuoles seen in the first 10 Tetrahymena cells were recorded for each slide. After the 10 Tetrahymena were found on each slide the slides were placed into a discard bin in the center of the table. The food vacuoles found on each slide were then averaged to get the mean number at each time point which was recorded in a separate table. …show more content…
200 microliters of the Dri-Contrad stock chemical, 200 microliters of the carbon solution, and 200 microliters of Tetrihymena were all added to a microcentrifuge tube labeled X-U. After 3, 6, and 9 minutes, 100 microliters of solution from the X-U tube was transferred to three tubes labeled 3X-U, 6X-U, and 9X-U. A wet mount was made of 50 microliters from each tube to their corresponding glass slide. Each slide was then observed under a dissecting microscope and the number of food vacuoles seen in the first 10 Tetrahymena cells were recorded and averaged to get the mean number of food vacuoles per cell at each time