Temperature is one thing that could affect the percentage the most but all the cows conducted in the tests were in the same environment. They also used other motives to get the best results possible,
The scientists preselected cows that were synchronized for ovulation, that had a follicle size between 12 and 18 mm, and clear vaginal discharge at the time of AI were inseminated. A shallow uterine insemination was performed into the uterine horn ipsilateral to the side of probable impending ovulation. (Karakaya et al, 2014)
The outcome of this experiment shown in the graphs below.
As seen in table three, the percentages are broke down even more by primiparous and multiparous cows to gain results that are more accurate. The reason why is,
Heifers have a much smaller uterus than do mature cows. The smaller physical size of the tract allows a lower dose of sorted sperm to complete the journey from the body of the uterus to the sight of fertilization in the oviduct. The uterus of a mature cow is two to five times larger than that of a heifer, which makes a low dose insemination into the cow less efficient. (Stroud, 2006)
The outcome of the experiment from the graphs show that there is a difference between the sex-sorted semen and the conventional