One hundred eleven samples of blood, tissue, hair, and other types of specimens were studied, characterized and hypothesized to be obtained from elusive hominins in North America commonly referred to as Sasquatch. DNA was extracted and purified from a subset of these samples that survived rigorous screening for wildlife species identification. Mitochondrial DNA (mtDNA) sequencing, specific genetic loci sequencing, forensic short tandem repeat (STR) testing, whole genome single nucleotide polymorphism (SNP) bead array analysis, and next generation whole genome sequencing were conducted on purported Sasquatch DNA samples gathered from various locations in North America. …show more content…
The sequences that were subjected to BLAST searches in GenBank®40 showed consistent homology with human haplotypes. No mitochondrial DNA homology with apes, Neanderthal or Denisova cave sequences were found.
The results showed that the mtDNA was human. The haplotypes were human. Blast searches showed that the sequences did not match Apes, Neanderthal, or Denisovan. Of the thirty samples twenty yielded whole mitochondrial sequences and ten yielded partial mitochondrial genomes. The thirty samples showed sixteen different human haplotypes.
The interesting and unexpected finding is the origin of the haplotypes. The majority of the haplotypes show origination from Europe, Asia, Middle East, and Africa. On only four of the haplotypes originated close to where the samples were collected in North America.
The North American haplotypes represented the following Native American Tribes:
• Haplotype A6L2c - Inuit and Nivkh peoples
• Haplotype C - Evenks and Yakuts …show more content…
The X chromosome failing to amplify, which is known as a “dropout”, was considered by Dr. Ketchum as the “most significant of the findings observed with the STR genotype analysis of amelogenin.” I explained earlier in this chapter about how a single source DNA sample with ample quantity, excellent quality, and purity will amplify correctly yielding extremely clear results. Dr. Ketchum had these types of samples. These “pristine” samples should have amplified and yielded superb results for the amelogenin gene. But some of these DNA samples did not amplify. Other DNA samples gave very strange results. This is a major indicator that the DNA is not from a completely human source and the subject is a human hybrid.
Seven samples only yielded a Y chromosome. Twelve of the samples that amplified and yielded human haplotypes failed to amplify for sex determination. This is very strange, but in my opinion to be expected from a hybrid creature like a Sasquatch.
Some of the samples indicated a normal human X chromosome, while others failed completely to amplify.
When the Y chromosome was amplified across Exons (exons are parts of DNA that are converted into mature messenger RNA) 1, 2, 4/5 and 8, varied results were obtained, with no samples successfully amplifying and sequencing across all five exons (except the human controls).
When the nuDNA sequences were compared to Genbank using a BLAST search, none of the nuDNA