significant change to the platelet count that has been established to be 10 µM. Such was confirmed by the flow cytometry that exhibited the highest increment in larger platelets, with increase ranging between 10 and 20 µM of ADP. However, 20 µM could not be used because it would cause excessive aggregation.
Platelets contained in population 1 (P1) area are all of the same size as that of the resting platelets and they are assumed not to be activated. Whereas, the platelets in population 2 (P2) area tend to be bigger. It is assumed that they are activated. As more agonists are added, the percentage of larger platelets increases. Such is likely to arise because as the platelets grow bigger, the more they scatter forward and sideways. In a graph showing change in size, it is possible for flow cytometer to detect a shift that takes place in the size of the platelets.
The platelet aggregation testing aim at measuring the …show more content…
Then, an addition of 5 µL of the diluted ADP was made for platelet stimulation and aggregometry reading made within the initial minute after the baseline is reached. The moment ADP was added, platelets were aggregated and activated even with limited light absorption. Therefore, the photocell detected an increase in light transmission. Before testing the samples, the calibration of the aggregometer was done by placing a cuvette that contained controls PRP equated to 0 percent light transmission while the other cuvette that contained PPP equated to 100 percent light transmission. Aggregation assessment was undertaken in four to five minutes for each sample. While reading, the instrument would read the maximum absorption (maxA), (Lag), (Slp) and area under curve through the use of Addrolink8 software version 1.2.8 and which contain 2
Platelets contained in population 1 (P1) area are all of the same size as that of the resting platelets and they are assumed not to be activated. Whereas, the platelets in population 2 (P2) area tend to be bigger. It is assumed that they are activated. As more agonists are added, the percentage of larger platelets increases. Such is likely to arise because as the platelets grow bigger, the more they scatter forward and sideways. In a graph showing change in size, it is possible for flow cytometer to detect a shift that takes place in the size of the platelets.
The platelet aggregation testing aim at measuring the …show more content…
Then, an addition of 5 µL of the diluted ADP was made for platelet stimulation and aggregometry reading made within the initial minute after the baseline is reached. The moment ADP was added, platelets were aggregated and activated even with limited light absorption. Therefore, the photocell detected an increase in light transmission. Before testing the samples, the calibration of the aggregometer was done by placing a cuvette that contained controls PRP equated to 0 percent light transmission while the other cuvette that contained PPP equated to 100 percent light transmission. Aggregation assessment was undertaken in four to five minutes for each sample. While reading, the instrument would read the maximum absorption (maxA), (Lag), (Slp) and area under curve through the use of Addrolink8 software version 1.2.8 and which contain 2