* For the first one this is what I would do... okay so we have the original equation for the first one & based off that this is what I would do... I would discover f ( 2 ) by inserting x equals 2. f…
Lab #5: Introduction to Metasploit on Kali Linux Team: CRYPTERS 1 d. Why is it usually a bad idea to operate in the Linux environment as root? If you are unfamiliar with the concept of the root user, do a quick google search. It is always a good practice on any operating system to run your applications on a user level and leave the administrative tasks to the root user, and only on a per-need basis. Applications are meant to be run by users with non-administrative privileges.(Power December 4, 2010)…
A sketch or view of test setup and arrangement of gauges and loading protocol should be presented. It is not clear the location of gauge 1 and 2 listed in Figure 11. Why the results of two mentioned gauges are presented in comparison with analytical one? Are the both of them comparable? Why the results of two gauges are different?…
The mutation 1 plasmid had a substitution mutation at the 173-base pair. The lab group used the bioinformatics program muscle to align the genetic codes of the two plasmids for comparison, which the group used a computer program called Putty to log in and view. The lab group assumed that lanes A, B, and E would move further down the hyperladder because they were uncut and supercoiled allowing them to travel further through the gel at a faster rate than those strands that were cut because of restriction enzymes. However, after further discussion between the lab group and the instructors, the lab group found that most plasmids had been nicked, uncoiling them but keeping them as a circular shape, making them much slower. So, the lab group decided to change their predictions that lanes A, B, and E would all be above the hyper ladder.…
Data Analysis Does microwave radiation increase the speed the plant is growing? There were more than one variable. The independent variable is the amount of microwave radiation the seed received. The control group is non-microwavable seed, which was cell #1. The experimental group is microwaved seeds.…
Now, the donor bacterium transfers genetic material to the recipient bacterium. During the process of conjugation, one of the bacterium aids as the donor of the genetic material. The other serves as the recipient. The donor bacterium carries a DNA sequence. One strand of the plasmid is transported to the other and the other remains in the original cell.…
These thymine dimers cause the normally linear reading polymerase to perceive the DNA as a bubble; either passing it entirely, or just coding the complementary strand incorrectly. The longer the exposure to UV light, the more inconsistencies in the genome.3 The basis for this experiment is to use UV mutation long enough to cause a Kanamycin resistance, by causing changes in several genes of interest, and producing a non-motile strain by mutations in several flagellar gene clusters. By using intervals of UV light, you can generate a UV killing curve, a model that allows you to select the amount of bacterial…
If the transformed E.coli bacterium are in the presence of arabinose, they form colonies that are able to glow green when held under UV light. The bacterium in the…
A plasmid is circular, relatively small in size, double stranded as a DNA molecule, and is physically distinct from the chromosomal DNA of a cell. Innately found in prokaryotic bacterial cells, plasmids can sometimes be found in eukaryotes as well 1. Plasmids are self-replicating; therefore, when a plasmid is formed, infinite copies of the plasmid can be created if the plasmid is grown in bacteria. 2 Because of the size of plasmids, which can range anywhere from one thousand base pairs up to hundreds of thousands of base pairs, they are relatively easy to work with as modern technology has made the process of both establishing and adjusting plasmids to incorporate the genetic material of interest rather easy.…
Using the P22 phage, a lysate was prepared for the transduction of chloramphenicol resistance from a resistant S. typhimurium LT2 strain to a sensitive strain of S. typhimurium. It is believed that P22 phages package 44 kb of chromosomal DNA at random with a 2% frequency and a 2% chance of homologous recombination events occurring (MIC302 lab manual). S. typhimurium has a chromosome length of approximately 4,857 kb, so it was calculated that 0.00036% is the percent chance that the gene for chloramphenicol resistance will be transduced into the recipient non-resistant S. typhimurium strain’s chromosome. Using an MOI of 0.01, it was determined that 7µl of phage and 100µl of the cells were needed for the transduction. Results of cell and phage growth on LB-CM media…
Like this procedure where no bacteria grows if transformation failed it would be easy to tell what went wrong by whether or not there are luminescent parts. This could be done by purifying proteins as we did in this lab to determine whether or not the luminescent protein is functional and if not transferring it via…
The gene for GFP can be changed in transformed cells by adding arabinose to the cells nutrient medium. Transformed cells will grow on plates with LB/amp, and will appear white under UV light on plates not containing arabinose. By conducting this experiment you can see genetic…
Once the inserts are identified, the targeted gene sequence needs to be isolated by flanking sequence (7) and the mutant needs to be confirmed. (2) However, without phenotypic analysis, the linkage between the produced phenotype and mutant is remaining unclear. Thus, it is essential to confirm that the changes in mutant phenotype are caused by the insertion. (2) Some of the methods available to make the confirmation is co-segregation with reference, or transgenic technology to reproduce an identical mutant from wide type.…
Our goal within the plasmid isolation lab was to isolate the bacterial plasmid without any damage to the DNA. The formation of the clear lysate was essential because it allowed us to isolate the bacterial cells from removed media and later expose the DNA. Resuspension solution helped to resuspend the cells, after centrifugation, and prepare the cells for lysis solution. The lysis solution functions to degrade the cell membrane and spill its components. Then the Alkaline Protease functioned as the inhibitor of any DNases and denatured dsDNA to ssDNA shortly, by disturbing H-bonds between bases.…
Phase Diagram and Phase Transformation Fig. 1 shows (FCC) Nickel-(BCC) Chromium binary phase diagram. The eutectic reaction takes place at 1345°C and the composition is 51% Cr and 49% Ni. The solid solubility of Cr in Ni is extensive which is evident from the phase diagram.…