Peroxidase Lab Report

The hypothesis was proven wrong by the timing and values of the graph. I believe this was caused by the enzyme having to break apart a heightened amount of substrates in the mixed test tubes. This caused the initial data to yield a quicker reaction (on the basest of browning in color) compared to the altered data. The independent variable of this experiment was time, while the dependent variable was the color as the reaction is completely dependent on the duration of time. There were numerous potentially sources of error during this experiment. The seconds between beginning the timer and recording data and the slight overestimation in substance amounts. This experiment could be improved by having precise measurements and using numerous catalysts during the experiment to test the speeds of the reaction. …show more content…
The lack of bubbles was likely caused by the lack of a solid object with oxygen to react to. Yet, this experiment should have yield bubbles as oxygen was still being released. If it were to be boiled, then the amount of bubbles would have heightened, but not by much. If an enzyme is not denatured or inhibited in any fashion or method, it levels off through saturation or continuous use and an abundance of products between enzymes and substrates. To explain the cause of peroxidase becoming inactive re-assayed, it was caused by it already being previously used during the reaction with proteolytic enzyme trypsin. Additionally, you cannot reuse it as there is nothing left of it to properly react with. The high amounts of peroxidase caused the hydrogen peroxide inhibit itself. There is a lot of peroxidase trying to react to a small supply of hydrogen peroxide, ultimately halting the