Fig. 3. Stages of ovarian development in female Q-fly. Stage 1 and 2 represent previtellogenesis development. Stage 3 marks the initiation of vitellogenesis, and Stage 4 indicates late vitellogenesis. Stage 5 ovarian follicles had mature eggs.
Stages 1 and 2 are previtellogenic, while stages 3–4 represent the vitellogenic phase. Flies were assigned to stage 5 if the most advanced ovarian follicles had mature eggs. We did not observe stage 6, which is defined by a yellow residual follicular relic, the corpus luteum.
2.4. Effect of methoprene and YH on sexual development
2.4.1. Experimental treatments
One hundred and sixty millilitres of pupae from a single batch were placed in a mesh cage for adult emergence (Mega view Bug dorm 44545, 47.5 x 47.5 x 47.5 cm). After 24 …show more content…
Effect of methoprene and YH on longevity
Application of treatments for longevity trials was similar to that of mating trials. Male and female flies were sorted within 3 days after emerging by transferring individual flies in glass tubes and placed in longevity cages (group and individual). No calling, courting, or mating were observed in cages prior to placing in the longevity trial cages. Longevity of flies was assessed in both group and individual cages.
In group cages, for each of treatment, five flies of either sex were placed in each of three 1.125 L cages having a mesh-covered window (ca. 28cm2) for ventilation (i.e., 15 male and 15 female flies for each treatment). All cages were provided with water-soaked cotton and sugar in a 35 mm Petri dish.
In individual cages, for each of treatments 10 male and 10 female flies were placed singly in 70ml containers having 8-10 2-mm holes for ventilation. Flies were provided with water-soaked cotton and sugar (as a solution dried onto 1-cm squares of porous paper). Flies were checked daily until all had died. Dead flies were removed from the cages daily. Both group and individual trials were repeated twice.
2.6. Statistical
Stages 1 and 2 are previtellogenic, while stages 3–4 represent the vitellogenic phase. Flies were assigned to stage 5 if the most advanced ovarian follicles had mature eggs. We did not observe stage 6, which is defined by a yellow residual follicular relic, the corpus luteum.
2.4. Effect of methoprene and YH on sexual development
2.4.1. Experimental treatments
One hundred and sixty millilitres of pupae from a single batch were placed in a mesh cage for adult emergence (Mega view Bug dorm 44545, 47.5 x 47.5 x 47.5 cm). After 24 …show more content…
Effect of methoprene and YH on longevity
Application of treatments for longevity trials was similar to that of mating trials. Male and female flies were sorted within 3 days after emerging by transferring individual flies in glass tubes and placed in longevity cages (group and individual). No calling, courting, or mating were observed in cages prior to placing in the longevity trial cages. Longevity of flies was assessed in both group and individual cages.
In group cages, for each of treatment, five flies of either sex were placed in each of three 1.125 L cages having a mesh-covered window (ca. 28cm2) for ventilation (i.e., 15 male and 15 female flies for each treatment). All cages were provided with water-soaked cotton and sugar in a 35 mm Petri dish.
In individual cages, for each of treatments 10 male and 10 female flies were placed singly in 70ml containers having 8-10 2-mm holes for ventilation. Flies were provided with water-soaked cotton and sugar (as a solution dried onto 1-cm squares of porous paper). Flies were checked daily until all had died. Dead flies were removed from the cages daily. Both group and individual trials were repeated twice.
2.6. Statistical