Material and Method
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The practical course of the present study was implemented in the farm ofFaculty of Agriculture,Minia University, Minia Governorate.
2.1. Animals:
The present study was conducted on twelve healthy Osemi rams, 4 months old, located at the farm of Faculty of Agriculture, Minia University. The body weight of rams was (17.6 ± 1.26 kg).
2.2. Ration:
Basal diet was formed from commercial normal diet, containing corn 42%, soybean 16% and bran 42% with adding 0.03% minerals, 0.5 % sodium chloride, 0.03% premix and probiotic. This mixture is represents16% crude proteins and 2446 kcal kgG1.
2.3. Boron:
Boric acid (BA) "H3PO3 "(Nasr company for chemicals, Egypt) as a source of boron was used.
2.4. Experimental design: …show more content…
Rams of the first control group (group C) fed basal dietadlibitum.The second group (group B),the rams fed adlibitum the same basal diet supplemented with400 mgBA/kg dietwhich represent 70 mg B/kg diet.The rams in both groups fed their corresponding ration for 4 months till the rams reach 8 months old (2 months after age of maturity).
2.5. Measurement of total body weight:
The rams were weighted every month using electric balance. The live body weight changes were taken as a measure for growth.
2.6. Blood …show more content…
(1987). This test is a solid phase competitive EILA Kit for the in vitro quantitative determination of tri-iodothyronine (T3) and tetra-iodotyrosine (T4) concentration in serum. The principle of this test depends on that the assay system utilizes a highly specific T3 monoclonal antibody bound to a polystyrene well coated with goat anti-mouse antibody and an enzyme-labeled analytic. Test sample, T3 antibody solution, and a buffer containing chemical blocking agents and T3-enzyme conjugate are added to each antibody coated well. The blocking agents, 8-anilino-1-naphthalene sulfuric acid (ANS) and sodium salicylate, cause a release of T3 from the serum binding proteins and allow the T3 to bind to the antibody-coated well. During 60 minute incubation, T3 in the sample sera competes with the T3-enzyme conjugate for binding sites on the coated wells. The numbers of binding sites on the well are limited; as more of them are occupied by T3 from the sample, less of the T3 enzyme conjugate can bind. The amount of T3 in the serum sample is inversely proportional to the amount of T3-enzyme conjugate bound to the well. After a short incubation, the wells are washed to remove any unbound T3-enzyme conjugate. An enzyme substrate-chromogen (hydrogen peroxide, H2O2, and tetramethyl benzidine, TMB) is added tothe well and incubated for 15 minutes at room temperature,
================
The practical course of the present study was implemented in the farm ofFaculty of Agriculture,Minia University, Minia Governorate.
2.1. Animals:
The present study was conducted on twelve healthy Osemi rams, 4 months old, located at the farm of Faculty of Agriculture, Minia University. The body weight of rams was (17.6 ± 1.26 kg).
2.2. Ration:
Basal diet was formed from commercial normal diet, containing corn 42%, soybean 16% and bran 42% with adding 0.03% minerals, 0.5 % sodium chloride, 0.03% premix and probiotic. This mixture is represents16% crude proteins and 2446 kcal kgG1.
2.3. Boron:
Boric acid (BA) "H3PO3 "(Nasr company for chemicals, Egypt) as a source of boron was used.
2.4. Experimental design: …show more content…
Rams of the first control group (group C) fed basal dietadlibitum.The second group (group B),the rams fed adlibitum the same basal diet supplemented with400 mgBA/kg dietwhich represent 70 mg B/kg diet.The rams in both groups fed their corresponding ration for 4 months till the rams reach 8 months old (2 months after age of maturity).
2.5. Measurement of total body weight:
The rams were weighted every month using electric balance. The live body weight changes were taken as a measure for growth.
2.6. Blood …show more content…
(1987). This test is a solid phase competitive EILA Kit for the in vitro quantitative determination of tri-iodothyronine (T3) and tetra-iodotyrosine (T4) concentration in serum. The principle of this test depends on that the assay system utilizes a highly specific T3 monoclonal antibody bound to a polystyrene well coated with goat anti-mouse antibody and an enzyme-labeled analytic. Test sample, T3 antibody solution, and a buffer containing chemical blocking agents and T3-enzyme conjugate are added to each antibody coated well. The blocking agents, 8-anilino-1-naphthalene sulfuric acid (ANS) and sodium salicylate, cause a release of T3 from the serum binding proteins and allow the T3 to bind to the antibody-coated well. During 60 minute incubation, T3 in the sample sera competes with the T3-enzyme conjugate for binding sites on the coated wells. The numbers of binding sites on the well are limited; as more of them are occupied by T3 from the sample, less of the T3 enzyme conjugate can bind. The amount of T3 in the serum sample is inversely proportional to the amount of T3-enzyme conjugate bound to the well. After a short incubation, the wells are washed to remove any unbound T3-enzyme conjugate. An enzyme substrate-chromogen (hydrogen peroxide, H2O2, and tetramethyl benzidine, TMB) is added tothe well and incubated for 15 minutes at room temperature,