• Skin prick tests are usually performed on the inner forearm. Any number of allergens can be tested, as few as 3 or 4 or up to about 25 allergens. The tested allergens in our study were sodium benzoate and potassium sorbate.
• The arm is cleaned with soap and water or alcohol
• The forearm is coded with a skin marker pen corresponding to the number of allergens being tested. Marks should be at least 2cm apart.
• A drop of allergen solution is placed beside each mark
A small prick through the drop is made to the skin using a sterile prick lancet. Excess allergen solution is dabbed off with a tissue.
In addition to the allergens tested, there should be a positive and negative control. The positive control, usually a …show more content…
These indications include assessment of the accuracy of other diagnostic methods, including skin tests, serum IgE measurement and patch test as well as assessment of the threshold doses of different food allergens. OFC can also be helpful in determination of the effects of food processing on the allergenicity of various food allergens. Lastly, it can be used to assess the efficacy of potential treatments of food allergy (Cochrane et al.,2012).
Procedure of open food challenge test:
1- The test will be explained to the patients. Antihistaminics, steroids, beta blockers and angiotensinogen converting enzyme inhibitor (ACEI) should be stopped. 2- A cannula will be inserted ,antihistaminics and I.V. steroids will be kept ready for any anaphylactic reaction. 3- One ml of the tested additive will be administered by ingestion to each patient, close monitoring for 15-30 minutes, then the dose is doubled if no signs appeared. 4- Monitoring before doubling dose includes measuring respiratory rate, pulse, blood pressure,checking for skin manifestations or any abdominal pain. 5- Monitoring remains for 12 hours at hospital,on discharge patients will be informed about any signs of allergy if developed at home within the next 12 hours (Bindslev-Jensen et al., …show more content…
Patients who manifested mild symptoms such as a few hives that resolved promptly with or without treatment might be discharged after a 2 hour observation period after resolution of symptoms. Patients with minimal residual symptoms such as a few new hives or swollen lips may be sent home after 4 hours, whereas a patient with a past history of a severe reaction should be observed for a longer period even in the absence of