After incubation for the SIM test the Kovac’s reagent was added and the top of the broth did not turn a dark red, it was decided that this was a negative test for Indole. With the negative Indole test result this eliminates E. coli and P. vulgaris. The last test I did was use a Urea broth test for #29. The results from this test were negative which means there was no production of urease to break down urea and produce a pH change to trigger the phenol red to turn pink. This outcome eliminated Klebsiella pneumonia as a possibility. The Urea test indication concluded that the Unknown #29 was Enterobacter aerogenes. But for good measure as a Final test I used the Methyl Red-Vogues Proskauer test to double check my results. The MR-VP test was performed, giving the expected negative result. This indicated that the unknown did not produce acids as a byproduct of glucose metabolism. The negative result eliminated K. pneumoniae from the list of remaining possibilities. The Voges-Proskauer test demonstrated a positive result indicating that Enterobacter aerogenes was the unknown bacterium. The positive test indicated that the unknown, E. aerogenes does not produce acetyl methyl
After incubation for the SIM test the Kovac’s reagent was added and the top of the broth did not turn a dark red, it was decided that this was a negative test for Indole. With the negative Indole test result this eliminates E. coli and P. vulgaris. The last test I did was use a Urea broth test for #29. The results from this test were negative which means there was no production of urease to break down urea and produce a pH change to trigger the phenol red to turn pink. This outcome eliminated Klebsiella pneumonia as a possibility. The Urea test indication concluded that the Unknown #29 was Enterobacter aerogenes. But for good measure as a Final test I used the Methyl Red-Vogues Proskauer test to double check my results. The MR-VP test was performed, giving the expected negative result. This indicated that the unknown did not produce acids as a byproduct of glucose metabolism. The negative result eliminated K. pneumoniae from the list of remaining possibilities. The Voges-Proskauer test demonstrated a positive result indicating that Enterobacter aerogenes was the unknown bacterium. The positive test indicated that the unknown, E. aerogenes does not produce acetyl methyl