Mitochondria and caspases are essential elements of the controlling mechanism of apoptosis 1. Caspases, a family of enzymes that are key effectors of apoptosis, are cysteine proteases that cut after aspartic acid residues. Several of these enzymes, like caspase 3 and caspase 7, are responsible for many of the effects of apoptosis. These effects include cell shrinkage, PS externalization, and membrane microvesiculation 2-5.
Cytochrome C, a component of the electron transport chain, is normally located in mitochondrial intermembrane space, and its release into the cytosol is generally the earliest and most critical initiating factor for mitochondrial mediated apoptosis 6-7.
Platelets express several distinctive apoptotic signs during storage 8-10. PS exposure is also a characteristic of apoptosis and appears to be a mechanism by which apoptotic cells are recognized and …show more content…
In living cells, it is required for the transport of fatty acids from the intermembraneous space in the mitochondria, into the mitochondrial matrix during the breakdown of fats and the generation of metabolic energy 20-21.
LC modulates platelet activation through the antioxidant mechanism. More recently, a role for the LC in the maintenance of membrane integrity has been suggested 22-23. LC has been found to reduce apoptosis of rat bone marrow cell cultures, lymphocyte, and primary cultured neurons 24-27.
In our previous study, we demonstrated that LC could be used as an additive material for stored PC to improve the platelet metabolism, quality and survival, during 5-day storage 28. However, the effect of L-carnitine on platelet apoptosis remains unknown. The aim of this study was to investigate the effects of LC on platelet apoptosis via studying different parameters like the mitochondrial enzyme activity and mitochondrial membrane potential during storage
Cytochrome C, a component of the electron transport chain, is normally located in mitochondrial intermembrane space, and its release into the cytosol is generally the earliest and most critical initiating factor for mitochondrial mediated apoptosis 6-7.
Platelets express several distinctive apoptotic signs during storage 8-10. PS exposure is also a characteristic of apoptosis and appears to be a mechanism by which apoptotic cells are recognized and …show more content…
In living cells, it is required for the transport of fatty acids from the intermembraneous space in the mitochondria, into the mitochondrial matrix during the breakdown of fats and the generation of metabolic energy 20-21.
LC modulates platelet activation through the antioxidant mechanism. More recently, a role for the LC in the maintenance of membrane integrity has been suggested 22-23. LC has been found to reduce apoptosis of rat bone marrow cell cultures, lymphocyte, and primary cultured neurons 24-27.
In our previous study, we demonstrated that LC could be used as an additive material for stored PC to improve the platelet metabolism, quality and survival, during 5-day storage 28. However, the effect of L-carnitine on platelet apoptosis remains unknown. The aim of this study was to investigate the effects of LC on platelet apoptosis via studying different parameters like the mitochondrial enzyme activity and mitochondrial membrane potential during storage