Identifying the Presence of Macromolecules Using Benedict’s, Biuret’s, Sudan III, and Lugol’s Solution
Betty Ngo
Introduction
Macromolecules are the critically important large molecules that fall into four main classes: carbohydrates, lipids, proteins and nucleic acids. Macromolecules are polymers which are built up by monomers. Monomers become polymers from the process dehydration reaction and polymers are broken apart by the process hydrolysis. Macromolecules are critically important to all living organisms because they perform basic functions necessary for an organism to live.
Firstly, carbohydrates, constructed from polysaccharides, are used to store energy or for structure. For example, synthesizing starch enables plants …show more content…
To test for monosaccharides, a 3-mL of Benedict’s solution was added to a new 5-mL sample of cheeseburger slushie. It was heated in a beaker of boiling water for five minutes until the mixture became orange. To test for starches, 5 drops of Lugol’s solution was added to a 5-mL sample of the cheeseburger slushie. To test for proteins, 10 drops of Biuret’s reagent was added to another 5-mL cheeseburger slushie and lastly, to test for lipids, 5-mL of water was added to 5-mL of the slushie. Then, 5 drops of Sudan III was added. This process was repeated with the McNugget slushie. The test tube was cleaned after every test was …show more content…
The results were the same for both the cheeseburger meal and the McNugget meal. There was a negative result for protein presence from the Biuret test.
Conclusion
From the data presented, it does not support the hypothesis. There is a presence of monosaccharaides, starches, and lipids however there was no presence of proteins. This was odd because the meat products, such as chicken or beef, should’ve provided protein in the meal. However, due to this test, the results were negative perhaps because the peptide bonds were broken in the process of creating the slushie. There could have been some errors during the procedure that could have produced erroneous results such as inaccurate measurements of the slushie or improper heating techniques during the monosaccharide test. The presence of lipids could very much possibly come from the oil that was used for the fries, the glucose or carbohydrates came from the soda, and the starch presence came from the
Betty Ngo
Introduction
Macromolecules are the critically important large molecules that fall into four main classes: carbohydrates, lipids, proteins and nucleic acids. Macromolecules are polymers which are built up by monomers. Monomers become polymers from the process dehydration reaction and polymers are broken apart by the process hydrolysis. Macromolecules are critically important to all living organisms because they perform basic functions necessary for an organism to live.
Firstly, carbohydrates, constructed from polysaccharides, are used to store energy or for structure. For example, synthesizing starch enables plants …show more content…
To test for monosaccharides, a 3-mL of Benedict’s solution was added to a new 5-mL sample of cheeseburger slushie. It was heated in a beaker of boiling water for five minutes until the mixture became orange. To test for starches, 5 drops of Lugol’s solution was added to a 5-mL sample of the cheeseburger slushie. To test for proteins, 10 drops of Biuret’s reagent was added to another 5-mL cheeseburger slushie and lastly, to test for lipids, 5-mL of water was added to 5-mL of the slushie. Then, 5 drops of Sudan III was added. This process was repeated with the McNugget slushie. The test tube was cleaned after every test was …show more content…
The results were the same for both the cheeseburger meal and the McNugget meal. There was a negative result for protein presence from the Biuret test.
Conclusion
From the data presented, it does not support the hypothesis. There is a presence of monosaccharaides, starches, and lipids however there was no presence of proteins. This was odd because the meat products, such as chicken or beef, should’ve provided protein in the meal. However, due to this test, the results were negative perhaps because the peptide bonds were broken in the process of creating the slushie. There could have been some errors during the procedure that could have produced erroneous results such as inaccurate measurements of the slushie or improper heating techniques during the monosaccharide test. The presence of lipids could very much possibly come from the oil that was used for the fries, the glucose or carbohydrates came from the soda, and the starch presence came from the