Congo red and destained with 1M sodium chloride for 15 min. The clear halo was observed around the colony indicates the cellulase activity.
Lipase detection (Samad et al. 1989)
The lipase activity was performed by growing the isolates on the peptone agar media (10g peptone, 16g agar, 1000 ml distilled water, pH 7.2) amended 10 ml Tween 80 and 0.01% phenol red (as an indicator). This media was inoculated with the isolates and incubated. The change of media color surrounding the colony from red to yellow was considered positive for lipase enzyme
Lipase detection (Samad et al. 1989)
The lipase activity was performed by growing the isolates on the peptone agar media (10g peptone, 16g agar, 1000 ml distilled water, pH 7.2) amended 10 ml Tween 80 and 0.01% phenol red (as an indicator). This media was inoculated with the isolates and incubated. The change of media color surrounding the colony from red to yellow was considered positive for lipase enzyme