Lactose intolerance is one of the most common conditions, which affects millions of people. People with this condition do not produce the lactase enzyme which metabolizes the sugar on their own therefore they are unable to break down the Lactose which is sugar that is found in milk. Lactase is an enzyme found specifically in milk sugar “Lactose”. It breaks down the lactose into monosaccharaides, galactose and glucose. The Lactase enzyme can be used to aid people with this condition.
Saladin, K. (n.d.). ANATOMY & PHYSIOLOGY: THE UNITY OF FORM AND FUNCTION BUNDLE Edition: 7TH. McGraw-Hill Education; 7.
Hypothesis: When we add the enzyme solutions at different temperatures it can alter the function. We will be able to test …show more content…
2ml of milk along with 1ml of enzyme solution at room tempurature were added to test tube #1. In test tube #2, 2ml of milk with 1ml of denatured (heated) enzyme solution were added. In test tube #3 both milk and water were added. In test tube #4 a sucrose solution along with the enzyme solution at room temperature were added. In test tube #5 the sucrose solution with the denatured (heated) enzyme solution were added. Finally in test tube #6 the sucrose solution and milk were added. Once the two-minute time frame had passed all of the test tube where then tested for the presence of glucose. The data was recorded in a table. If glucose was present we labeled it with a “+” symbol and if it was negative we labeled it with a “-“ symbol.
Results:
Data Table for Lactase Enzyme Lab
Test Tube #1: Milk + Enzyme solution at room temperature +
Test Tube #2: Milk + Denatured enzyme solution -
Test Tube #3: Milk + Water -
Test Tube #4: Sucrose + Enzyme solution at room temperature -
Test Tube #5: Sucrose + Denatured enzyme solution -
Test Tube #6: Sucrose + Water -
All of the solutions were in the test tube for duration of 2 minutes and then tested with the glucose stick being placed in the solution for duration of 30seconds. Below you will find the