Effects of Temperature and Substrate on Catalyst Enzymes
Ryan Smith
Lab Section 030
Abstract In order to turn hydrogen peroxide into water and oxygen by using enzyme called a catalyst. To determine the effects of temperature and substrate concentrations we preformed an experiment testing the effects on the catalyst enzyme reaction. The hypothesis for our first experiment was if the substrate concentration is larger than the catalyst enzymes activity will be faster as well. The result followed suit with our first experiment and supported our hypothesis. The hypothesis for our second experiment was if the temperature is increased then the rate of the catalyst enzymes activity will be faster. By keeping the substrate concentration at a constant …show more content…
The set of for this experiment included filling a 500 ml beaker as well as a 10 ml test tube with water. the second step was to invert the test tube placing it in the beaker without allowing oxygen to enter the test tube. A piece of glass tubing connected to a rubber tube and stopper was then placed in the beaker. The side of the glass tube not attached to the rubber tube is placed under the open end of the test tube. Next 10 ml of the 0.8% concentration of substrate solution was added to 10 ml of the phosphate buffer and placed into a 50 ml Erlenmeyer flask. The flask opening was sealed off using the stopper attached to the opposite end of the rubber tube. The concentration in the flask was swirled until 10 ml of oxygen was collected in the test tube. The amount of time taken by the oxygen gas to fill the test tube replacing the water was recorded. After the initial experiment both the graduated cylinder and Erlenmeyer flask were thoroughly cleaned and the graduated cylinder was refilled with water. the graduated cylinder was placed back into the beaker of water and the glass tube was reinserted in the end. Next 10 ml of the 0.8% concentration of substance solution and 10 ml of the phosphate buffer and catalyst were added to the Erlenmeyer flask. The stopper was placed in the opening and swirled until 10 ml of O2 was captured in the graduated cylinder. The …show more content…
The amount of time take for the O2 to expel the water from the graduated cylinder. The data is categorized according to the following percentages of substrate .1%, .2%, .4%, .8%. The averages found from the results were 2.50 seconds, 6.34 seconds, 42.67 seconds, and 103.77 seconds. The calculated standard deviation for these averages were 0.43 seconds, 0.42 seconds, 14.99 seconds, and 55.62 seconds. The following graph, Graph 1, is a representation of the effect of change in substrate concentration on reaction rate. The line graph shows the correlation between these representations. The substrate concentration is shown by no catalyst, .8%, .4%, .2%, and .1%. the left side of the graph shows the
Ryan Smith
Lab Section 030
Abstract In order to turn hydrogen peroxide into water and oxygen by using enzyme called a catalyst. To determine the effects of temperature and substrate concentrations we preformed an experiment testing the effects on the catalyst enzyme reaction. The hypothesis for our first experiment was if the substrate concentration is larger than the catalyst enzymes activity will be faster as well. The result followed suit with our first experiment and supported our hypothesis. The hypothesis for our second experiment was if the temperature is increased then the rate of the catalyst enzymes activity will be faster. By keeping the substrate concentration at a constant …show more content…
The set of for this experiment included filling a 500 ml beaker as well as a 10 ml test tube with water. the second step was to invert the test tube placing it in the beaker without allowing oxygen to enter the test tube. A piece of glass tubing connected to a rubber tube and stopper was then placed in the beaker. The side of the glass tube not attached to the rubber tube is placed under the open end of the test tube. Next 10 ml of the 0.8% concentration of substrate solution was added to 10 ml of the phosphate buffer and placed into a 50 ml Erlenmeyer flask. The flask opening was sealed off using the stopper attached to the opposite end of the rubber tube. The concentration in the flask was swirled until 10 ml of oxygen was collected in the test tube. The amount of time taken by the oxygen gas to fill the test tube replacing the water was recorded. After the initial experiment both the graduated cylinder and Erlenmeyer flask were thoroughly cleaned and the graduated cylinder was refilled with water. the graduated cylinder was placed back into the beaker of water and the glass tube was reinserted in the end. Next 10 ml of the 0.8% concentration of substance solution and 10 ml of the phosphate buffer and catalyst were added to the Erlenmeyer flask. The stopper was placed in the opening and swirled until 10 ml of O2 was captured in the graduated cylinder. The …show more content…
The amount of time take for the O2 to expel the water from the graduated cylinder. The data is categorized according to the following percentages of substrate .1%, .2%, .4%, .8%. The averages found from the results were 2.50 seconds, 6.34 seconds, 42.67 seconds, and 103.77 seconds. The calculated standard deviation for these averages were 0.43 seconds, 0.42 seconds, 14.99 seconds, and 55.62 seconds. The following graph, Graph 1, is a representation of the effect of change in substrate concentration on reaction rate. The line graph shows the correlation between these representations. The substrate concentration is shown by no catalyst, .8%, .4%, .2%, and .1%. the left side of the graph shows the