There are several different levels at which a gene’s function can be controlled: at the level of chromatin, transcription, RNA-processing, translation, and even post- translationally. There are evolutionarily relevant reasons that might explain the levels at which different genes are controlled.
(3 points each) For each of the following scenarios, indicate which level of gene control you might expect a cell to use and a brief statement of why this type of control makes the most sense (no more than 25 words per scenario).
1) A gene in bacteria that responds to rapid changes in temperature
Transcriptional level to allow the bacteria to code for genes it needs to live in the new environment and turn off those it doesn’t need. …show more content…
9) What do the columns and rows represent? Each row represents a protein and the columns represent the tumor groups.
10) What genes (and how many) are included in this heatmap? 306 of the Intrinsic/UNC genes were used in the heatmap.
11) How was this set of genes selected out of all possible genes?
The researcher used breast tumor genes where they specifically picked samples that followed 70% of their criteria.
12) What samples were being compared within an individual microarray? Within an individual microarray, the proteins in “HER2+/ER-, Basal-like, LumA, LumB and Normal Breast-like tumor groups” are separately looked at.
13) What samples were being compared between microarrays? Between microarrays, the tumor groups are being compared to one another.
14) What molecules were actually molecularly labeled with dyes?
Proteins in each microarray were labeled with dyes. (Want me to be more specific?)
15) Name the specific colored dyes that were used? What color is each? Cy3 and Cy5 were the dyes used. Cy3 is orange and Cy5 is red.