Vitamins are the organic compounds required to carry out a living body’s metabolism and their absence can clump several metabolic reactions in the body. These essential nutrients cannot be synthesized in the body therefore must be taken from an outside food source i.e. plants, fruits, vegetables or animals. Basically there are two types of vitamins in nature; some are water soluble and others are the fat soluble ones. Water soluble vitamins are those which are soluble only in water and are B vitamins which include vitamin B6, vitamin B12, Niacin, folic acid, pantothenic acid, Thiamin (Vitamin B1), Riboflavin(Vitamin B2) and vitamin C(Ascorbic acid). These vitamins are first absorbed into the intestine and then passed to the blood and then
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Though there should not be high temperature for it as it is seen that it becomes the cause for the loss of vitamin D up to 20% or more in milk. Therefore for the extraction of vitamin D a mixture of petroleum ether and diethyl ether is mixed in an equal ratio, but vitamin A also got extracted with it, so to get pure vitamin D we should prepare a mixture of (6:4) ratio of ethanol with water and results showed to be prominent and a high quantity of vitamin D is extracted. Moreover vitamin E can also be extracted from this method, but by hot saponification. Vitamin E can be extracted from meat by adding methanol (KOH) and also ascorbic acid, and then being heated up to 80 to 90 degree Celsius for 45 minutes. The sample is left to cool and after that hexane/toluene mixture is added and sample is being shaken for 10 minutes and then also being centrifuged so that layer separation occurs and vitamin E is easily …show more content…
Actually the solvent breaks the lipoproteins bonds. For the lipid lineage a mixture of methanol and chloroform in ratio of (2:1) is used. If doing for milk then treat the milk with an ammonia solution and also alcohol and then cool it then extract with petroleum ether mixture. This results in protein precipitation which is soluble in ammonia and fat is extracted from this method. For vitamin A Hexane is used as the solvent and after the addition of water centrifugation is done which separates out layers and upper layer contains vitamin A with hexane which is then
Though there should not be high temperature for it as it is seen that it becomes the cause for the loss of vitamin D up to 20% or more in milk. Therefore for the extraction of vitamin D a mixture of petroleum ether and diethyl ether is mixed in an equal ratio, but vitamin A also got extracted with it, so to get pure vitamin D we should prepare a mixture of (6:4) ratio of ethanol with water and results showed to be prominent and a high quantity of vitamin D is extracted. Moreover vitamin E can also be extracted from this method, but by hot saponification. Vitamin E can be extracted from meat by adding methanol (KOH) and also ascorbic acid, and then being heated up to 80 to 90 degree Celsius for 45 minutes. The sample is left to cool and after that hexane/toluene mixture is added and sample is being shaken for 10 minutes and then also being centrifuged so that layer separation occurs and vitamin E is easily …show more content…
Actually the solvent breaks the lipoproteins bonds. For the lipid lineage a mixture of methanol and chloroform in ratio of (2:1) is used. If doing for milk then treat the milk with an ammonia solution and also alcohol and then cool it then extract with petroleum ether mixture. This results in protein precipitation which is soluble in ammonia and fat is extracted from this method. For vitamin A Hexane is used as the solvent and after the addition of water centrifugation is done which separates out layers and upper layer contains vitamin A with hexane which is then