Coli is therefore no exception. Those enzymes carry out many different functions in the cell and are of vital importance, so their pH sensitivity puts cells at a great risk when confronted by a change in pH. This is due to the covalent bonds between amino acids that have a positive charged part and another negative one. Oppositely charged areas have to match with each other when the substrate binds to the active site, and when the solution becomes too basic, a large number of hydroxide ions will bond to the positive charges of either the substrate or active site, preventing a perfect matching charge and making the enzyme less efficient and sometimes totally inactive. Furthermore, as the solution becomes more alkaline and more negative charges are present, the enzyme will eventually become denatured and thus lose the 3D shape and leading to a dysfunction in their respective function. In the end, the inhibition of enzymes will cause the death of E. coli bacterium as the proteins that are essential for bacterial growth will clump together and thus become inactivated, just like heat does, and kill
Coli is therefore no exception. Those enzymes carry out many different functions in the cell and are of vital importance, so their pH sensitivity puts cells at a great risk when confronted by a change in pH. This is due to the covalent bonds between amino acids that have a positive charged part and another negative one. Oppositely charged areas have to match with each other when the substrate binds to the active site, and when the solution becomes too basic, a large number of hydroxide ions will bond to the positive charges of either the substrate or active site, preventing a perfect matching charge and making the enzyme less efficient and sometimes totally inactive. Furthermore, as the solution becomes more alkaline and more negative charges are present, the enzyme will eventually become denatured and thus lose the 3D shape and leading to a dysfunction in their respective function. In the end, the inhibition of enzymes will cause the death of E. coli bacterium as the proteins that are essential for bacterial growth will clump together and thus become inactivated, just like heat does, and kill