How Does Gibberellic Acid Affect Germination

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Two different types of seeds, Radish and Cactus, were grown using different concentrations of Gibberellic acid, to see how it would affect their germination time. The amount of germinated seeds were counted each day, and then compared with the combined data from the rest of the class.
Gibberellic acid is a metabolic byproduct of the “Gibberella Fujikuroi” fungus. It caused the stems of rice to grow more rapidly than usual, so rapidly that the plant would collapse (Riley 1987) (Gupta, Chakrabarty 2013). Gibberellic acid is a very potent hormone, its natural occurrences controls the development in plants. Too much Gibberellic acid may have the opposite effect and too little may require the plant to be repeatedly treated with the acid (Riley 1987).
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Hypothesis is, if the concentration of Gibberellic acid available to the seed increased, then the time for the seed to germinate would decrease. The independent variable was the concentration of Gibberellic acid used. The dependant variable was the amount of seeds that had germinated. Controlled variables were, the amount of seeds in each bag, the amount of solution used in each bag, the type of seeds used, and the amount of sunlight reaching the seeds.

First the Radish seeds were counted out and divided into groups of 10. Each concentration of Gibberellic acid was added to two paper towels, for reliability, and then each was placed into a ziplock bag. Then, 10 radish seeds were placed into each of 10 zip lock bags. All previous steps were then repeated with Cactus seeds. All 20 bags were labeled with the type of seed and the concentration of Gibberellic acid inside. Each day the bags were inspected and the amount of germinated seeds was counted and
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If two seeds are too close together than one seed will not germinate while the other one will. This could have affected the experiment, because if the seeds were too close together, than some would not germinate, irrelevant of the concentration of gibberellic acid. This could have been reduced by using larger bags. Another possible issue with the experiment is a small sample size. There was only two replicates for each concentration. This was mitigated through the use of the combined class data. The combined data presents another potential problem, Human error. Human error could have been involved when using the combined class data because, different people may consider when a seed has germinated differently, for example, one person might say that t has germinated when the seed splits, while someone else might say that it hasn’t germinated until they can see the sapling turning green. Human error was mitigated during the smaller sample by having one person count the seeds each day. Another possible error in the experiment is random error, for example a seed could already be dead, this will skew the results because this seed will never germinate. Random errors can be reduced by having a larger sample

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