Purpose
This experiment consisted of passaging or subculturing a flask of HeLa cells. This provides more room for cells to grow. The process included transferring the cells into a new flask in order for them to continue growth, using trypsin to dissociate adherent cells from the flask, which then makes it possible to neutralize the trypsin and do a cell count. In addition, we used phenol red as our indicator of successful buffering.
It is important to note that the in vitro environment of cells needs to imitate the in vivo environment. Living conditions required for growing the cells in vitro include mimicking the physiological conditions of five factors such as nutrients, temperature, pH, growth surface, and gas environment (5-10% CO2). …show more content…
In Part B which was the analysis of cell-cell adhesion in suspension, the purpose of this part of the experiment was to compare cell growth and morphology on different surfaces and to see if our cell line would form …show more content…
In Part A, without these cell proliferation proteins, the cells would not be stimulated to grow. Moreover, cells were growing on a plastic surface in Part A whereas in Part B the surface was coated with agarose which prevents cell attachment. In general, our hypothesis did seem to be supported by the results seeing as media with serum did have a positive effect on the cells, especially for cell attachment. Furthermore, the hypothesis was supported by the fact that with the IDMEM+ after 24 hours we saw clumping and the forming of
This experiment consisted of passaging or subculturing a flask of HeLa cells. This provides more room for cells to grow. The process included transferring the cells into a new flask in order for them to continue growth, using trypsin to dissociate adherent cells from the flask, which then makes it possible to neutralize the trypsin and do a cell count. In addition, we used phenol red as our indicator of successful buffering.
It is important to note that the in vitro environment of cells needs to imitate the in vivo environment. Living conditions required for growing the cells in vitro include mimicking the physiological conditions of five factors such as nutrients, temperature, pH, growth surface, and gas environment (5-10% CO2). …show more content…
In Part B which was the analysis of cell-cell adhesion in suspension, the purpose of this part of the experiment was to compare cell growth and morphology on different surfaces and to see if our cell line would form …show more content…
In Part A, without these cell proliferation proteins, the cells would not be stimulated to grow. Moreover, cells were growing on a plastic surface in Part A whereas in Part B the surface was coated with agarose which prevents cell attachment. In general, our hypothesis did seem to be supported by the results seeing as media with serum did have a positive effect on the cells, especially for cell attachment. Furthermore, the hypothesis was supported by the fact that with the IDMEM+ after 24 hours we saw clumping and the forming of