3. RESULTS
3.1. Patients and controls
All patients and controls were in Hardy–Weinberg equilibrium ()
The rs243324 polymorphism showed evidence of Hardy–Weinberg disequilibrium in the patients and controls in total (P¼0.001) but not in the children in ALSPAC (Table 1), this was due to a slight excess in heterozygotes compared with the number expected
The mean age of disease onset was 29.5 (ranging from 17-46) years, and the mean duration of disease was 5.46 (ranging from 1-31) years. Some clinical features of patients and controls were presented in Table 1. The patients whom resided in Sistan and Baluchistan province for generations, aged between 19-55 years and only treated with Cinnovex were included while the patients treated with other drugs, migrate from other provinces, hospitalized at the time of study, pregnant females and any patient and control who had any inflammation situation were excluded. Also, patients and controls were matched by age, gender and time of blood sampling.
3.2 Gene expression
Statistical analysis of Ct (Cycle threshold) and ΔCt values revealed that a significant difference between the expression of SOCS1 gene in MS patients versus healthy controls (p=0.0001). Using 2-ΔΔCt method, our results revealed that the expression …show more content…
We performed this analysis for female group, whereas we found the association of rs243324 SNP in females. Finally, our results revealed that presence of rs243324 T allele in SOCS1 gene did not affect the expression level of SOCS1 mRNA in PBMCs of RR-MS patients (p= 0.18), controls (p= 0.9) and female groups (pcontrols= 0.61 and ppatients= 0.15) (figure 1). Unfortunately, we could not perform this analysis in the male group due to the low number of cases in MS and control groups of expression
3.1. Patients and controls
All patients and controls were in Hardy–Weinberg equilibrium ()
The rs243324 polymorphism showed evidence of Hardy–Weinberg disequilibrium in the patients and controls in total (P¼0.001) but not in the children in ALSPAC (Table 1), this was due to a slight excess in heterozygotes compared with the number expected
The mean age of disease onset was 29.5 (ranging from 17-46) years, and the mean duration of disease was 5.46 (ranging from 1-31) years. Some clinical features of patients and controls were presented in Table 1. The patients whom resided in Sistan and Baluchistan province for generations, aged between 19-55 years and only treated with Cinnovex were included while the patients treated with other drugs, migrate from other provinces, hospitalized at the time of study, pregnant females and any patient and control who had any inflammation situation were excluded. Also, patients and controls were matched by age, gender and time of blood sampling.
3.2 Gene expression
Statistical analysis of Ct (Cycle threshold) and ΔCt values revealed that a significant difference between the expression of SOCS1 gene in MS patients versus healthy controls (p=0.0001). Using 2-ΔΔCt method, our results revealed that the expression …show more content…
We performed this analysis for female group, whereas we found the association of rs243324 SNP in females. Finally, our results revealed that presence of rs243324 T allele in SOCS1 gene did not affect the expression level of SOCS1 mRNA in PBMCs of RR-MS patients (p= 0.18), controls (p= 0.9) and female groups (pcontrols= 0.61 and ppatients= 0.15) (figure 1). Unfortunately, we could not perform this analysis in the male group due to the low number of cases in MS and control groups of expression