Gene Of Interest Lab Report

Upon completion of the PCR, the final product should be highly concentrated DNA corresponding to the gene of interest. We ran our gene of interest on gel electrophoresis to compare our gene of interest to a marker of known size to confirm the size of our genomic DNA. On the image of the gel (figure 1), the sample containing the DNA ran, but the sample containing the DNA ladder did not. As a result, an appropriate determination of the sizes cannot be made because there is no ladder to compare it to in order to confirm the size of the gene of interest, madh1. However, if the marker had run correctly and if the gene of interest was present, then there should have been a band farther down on the gel slightly below 1500 bp on the gel marker (figure 3) corresponding to approximately 1348 bp which is the size of the madh1 gene. …show more content…
One such method is to use the ratio of the absorbance at the λmax of nucleic acids to that of the absorbance at the λmax of proteins. If the isolated genomic DNA is relatively pure, then the A260/A280 ratio should be approximately around the range of 1.6 to 1.9. Based upon completion of the calculations, the A260/A280 ratio was found to be 1.877 (Table 1) indicating that the isolated genomic DNA is pure. However, not only can the absorbance max be used to measure the purity of the nucleic acids, but it can also be used to measure the concentration of genomic DNA. Using the calculation DNA (µg) = A260 x 50 µg/(1 A260 x 1 ml) x dilution factor x total sample volume (ml), the concentration of DNA was calculated to be 25