targeted to the skin but instead an antihistamine used to prevent balance related sickness, this might suggest why the use of the alternative lipid, DSPC, gave better results.
For sample CIN14, (Table 8) that no cholesterol was used, observed the formation of crystals rather than functional liposomes and the most extreme drop in EE% of 28.33% (from 35.55% to 7.00%). This emphasises the requirement for some degree of fluidity for a membrane to be functional. As the presence of cholesterol is responsible for increased membrane fluidity, could assume little/no fluidity in the liposomes, hence they failed to form. Solid crystals were observed instead. It is observed that the cholesterol levels should not be increased too much however because, …show more content…
Gel beads are present on a column and, as the mixture (in this case liposomes) flows through the column, aspects of the mixture can be trapped via binding to, or entering, the gel beads. This occurs on a size related basis, with molecules of specific size being entrapped by the column (Ruysschaert et al., 2005). In the case of liposomes, their large size causes them to be entrapped between the gel beads (lots of gel must be present to stall the liposomes for a more hydrophobic environment) while the free cinnarizine, with its much smaller size, flows through. This means that the cinnarizine is eluted before the liposomes, allowing it to be collected in separate fractions (Akbarzadeh et al., 2013). In the future, gel filtration would use to separate the free cinnarizine from cinnarizine entrapped inside of liposomes (direct …show more content…
It adjusted the pressure applied on the liposomes as they were sensitive so their membranes may have been disrupted, leading to contents leakage. Shortening the centrifugation time also decreased the exposure of the liposomes to this pressure, decreasing the chance of membrane disruption. The method of centrifugation e.g. swinging head versus fixed angle, may also have affected the viability of the
For sample CIN14, (Table 8) that no cholesterol was used, observed the formation of crystals rather than functional liposomes and the most extreme drop in EE% of 28.33% (from 35.55% to 7.00%). This emphasises the requirement for some degree of fluidity for a membrane to be functional. As the presence of cholesterol is responsible for increased membrane fluidity, could assume little/no fluidity in the liposomes, hence they failed to form. Solid crystals were observed instead. It is observed that the cholesterol levels should not be increased too much however because, …show more content…
Gel beads are present on a column and, as the mixture (in this case liposomes) flows through the column, aspects of the mixture can be trapped via binding to, or entering, the gel beads. This occurs on a size related basis, with molecules of specific size being entrapped by the column (Ruysschaert et al., 2005). In the case of liposomes, their large size causes them to be entrapped between the gel beads (lots of gel must be present to stall the liposomes for a more hydrophobic environment) while the free cinnarizine, with its much smaller size, flows through. This means that the cinnarizine is eluted before the liposomes, allowing it to be collected in separate fractions (Akbarzadeh et al., 2013). In the future, gel filtration would use to separate the free cinnarizine from cinnarizine entrapped inside of liposomes (direct …show more content…
It adjusted the pressure applied on the liposomes as they were sensitive so their membranes may have been disrupted, leading to contents leakage. Shortening the centrifugation time also decreased the exposure of the liposomes to this pressure, decreasing the chance of membrane disruption. The method of centrifugation e.g. swinging head versus fixed angle, may also have affected the viability of the