Introduction: Gastro Intestinal Tract (GIT) is an important site for wide variety of lesions which include congenital, inflammatory, neoplastic conditions. The invention of flexible fiber-optic endoscope is a very good achievement towards the diagnostic field. With the use of this facility direct vision of diseased mucosa and biopsy of mucosal lesions, therapeutic induction and photographic visualization of the lesions are made possible.
Aim of the study: Aim of this study is to know the incidence of Gastro intestinal lesions with reference to age, sex and site and to study the histopathological types of Gastro Intestinal lesions.
Materials and Methods: The present study included 180 GIT biopsies (gastro intestinal biopsies) between the period August 2013 to August 2015 .Clinical details and case histories were obtained from the patients presented to the gastroenterology department. Biopsies were …show more content…
Biopsies were obtained by the gastroenterologist with fiber optic forward viewing endoscope. Rapid fixation was done to prevent shrinkage by placing the specimen in 10% neutral normal saline. Care was taken not to shake rigorously the bottle containing the specimen and also while transferring the tissue to the laboratory. After usual time fixation of the biopsy specimen, it was wrapped in a piece of filter paper after adding eosin and processed accordingly .It is then unwrapped and embedded in paraffin with mucosal surface uppermost .Four micron sections were cut perpendicular to this surface and 3 to 4 sections were taken on each slide .One slide was stained with Hematoxylin and Eosin stain and studied. Apart from the routine Hematoxylin and Eosin stain, the sections were studied with the following special stains wherever it was
Aim of the study: Aim of this study is to know the incidence of Gastro intestinal lesions with reference to age, sex and site and to study the histopathological types of Gastro Intestinal lesions.
Materials and Methods: The present study included 180 GIT biopsies (gastro intestinal biopsies) between the period August 2013 to August 2015 .Clinical details and case histories were obtained from the patients presented to the gastroenterology department. Biopsies were …show more content…
Biopsies were obtained by the gastroenterologist with fiber optic forward viewing endoscope. Rapid fixation was done to prevent shrinkage by placing the specimen in 10% neutral normal saline. Care was taken not to shake rigorously the bottle containing the specimen and also while transferring the tissue to the laboratory. After usual time fixation of the biopsy specimen, it was wrapped in a piece of filter paper after adding eosin and processed accordingly .It is then unwrapped and embedded in paraffin with mucosal surface uppermost .Four micron sections were cut perpendicular to this surface and 3 to 4 sections were taken on each slide .One slide was stained with Hematoxylin and Eosin stain and studied. Apart from the routine Hematoxylin and Eosin stain, the sections were studied with the following special stains wherever it was