3.4 Results & Discussion
3.4.1 The growth ofGlaciozyma antarctica PI12
The growth curve of G. antarctica PI12 (Figure 3.1) showed that the exponential phase of G. antarctica PI12 took place over 48 hours (2 days) and reached the stationary phase at 144 hours (6 days). The plotting of the diluted curve (red line) supported the curve of the original sample (blue curve) where both lines sharing the similar pattern. Similar steps were carried out using the original culture as well as the 1.5 diluted samples (Figure 3.2). Figure 3.1: This figure indicated the growth of G. antarctica PI12 in Yeast Peptone Dextrose (YPD) at 12oC shaking at 180rpm for 7 days. The blue line indicated growth curve plotted using the mean OD600nm of G. antarctica PI12 and the …show more content…
In this study, we employed the following formula to determine the cell doubling time.
(Time final – time initial (hours)) /(1.44 In (OD600nm final/OD600nm initial))
The final time we used was 96 hours; the initial time was 24 hours; OD600nm final was 1.428507 (average or three biological replicates); initial OD600nm was 0.062607 (average of three biological replicates). The OD600nm measurement of each sample is attached in Appendix B.
From the result, only Sample 3 (diluted 1:1) (Figure 3.4) showed that the cell count doubled after 24 hours, whereas the rest of the samples did not show a significant increase of cell count. This experiement needed to be improved and modified as the cultivation of G. antarctica PI12 in a hemocytometer might limit the growth and thus affect the result. In addition, Figure 3.5 indicates the cell count result obtained from the cell counter TC10, Bio-Rad. From the result, the cells multiplied from 10 million cells to 20 million cells within 10 hours (~70-80 hour in Figure
3.4.1 The growth ofGlaciozyma antarctica PI12
The growth curve of G. antarctica PI12 (Figure 3.1) showed that the exponential phase of G. antarctica PI12 took place over 48 hours (2 days) and reached the stationary phase at 144 hours (6 days). The plotting of the diluted curve (red line) supported the curve of the original sample (blue curve) where both lines sharing the similar pattern. Similar steps were carried out using the original culture as well as the 1.5 diluted samples (Figure 3.2). Figure 3.1: This figure indicated the growth of G. antarctica PI12 in Yeast Peptone Dextrose (YPD) at 12oC shaking at 180rpm for 7 days. The blue line indicated growth curve plotted using the mean OD600nm of G. antarctica PI12 and the …show more content…
In this study, we employed the following formula to determine the cell doubling time.
(Time final – time initial (hours)) /(1.44 In (OD600nm final/OD600nm initial))
The final time we used was 96 hours; the initial time was 24 hours; OD600nm final was 1.428507 (average or three biological replicates); initial OD600nm was 0.062607 (average of three biological replicates). The OD600nm measurement of each sample is attached in Appendix B.
From the result, only Sample 3 (diluted 1:1) (Figure 3.4) showed that the cell count doubled after 24 hours, whereas the rest of the samples did not show a significant increase of cell count. This experiement needed to be improved and modified as the cultivation of G. antarctica PI12 in a hemocytometer might limit the growth and thus affect the result. In addition, Figure 3.5 indicates the cell count result obtained from the cell counter TC10, Bio-Rad. From the result, the cells multiplied from 10 million cells to 20 million cells within 10 hours (~70-80 hour in Figure