Francisella Tularensis Essay

Background: Francisella tularensis (F.tularensis) is the etiological microorganism for tularemia. There are different forms of tularemia such as respiratory tularemia. Respiratory tularemia is the most severe form of tularemia with a high rate of mortality; if not treated. Therefore, traditional microbiological tools and Polymerase Chain Reaction (PCR) are not useful for a rapid, reliable, accurate, sensitive and specific diagnosis. But, DNA microarray technology does. DNA microarray technology needs to appropriate microarray probe designing.
Objective: The main goal of this original article was to design 15 microarray probes as the best candidates.
Method: For performing this research, the complete genomes of F.tularensis subsp. tularensis …show more content…
Besides, F.philomiragia is another species within the genus. However, there are considerable similarities between species and subspecies (1, 2, 29-32).
For this reason, the authors have used GView Server (https://server.gview.ca/) to show unique sequences in F.tularensis subspecies and F.philomiragia (Figure 1) (24, 25, 33, 34).
Regarding to GView Server application, the "unique genome" was selected for analysis type. Then the .gbk file belonging to F.tularensis subsp. tularensis FSC198 chromosome complete genome was downloaded from NCBI FTP site in FASTA format (ftp://ftp.ncbi.nlm.nih.gov/genomes/Bacteria/Francisella_tularensis_FSC198_uid58693/NC_008245.gbk), zipped and uploaded as a reference genome (24, 25, 33-35).
Then, the downloaded .fna files belonging to F.tularensis subsp. holarctica LVS (Type B), F.tularensis subsp. mediasiatica, F.tularensis subsp. novicida (F.novicida U112), and F.philomiragia subsp. philomiragia ATCC 25017 from (ftp://ftp.ncbi.nlm.nih.gov/genomes/Bacteria/) were also zipped and uploaded for comparing their genomes with the genome of F.tularensis subsp. tularensis FSC198 (Figure 1). All of the parameters in GView Server were set up as default position of the server (24, 25, 33, 35,