Bacterial Transformation with Escherichia coli
Byanka Delgado
BIO 181 Lab Thursday 1:30-4:15
Arizona State University
Abstract The major objectives of the Bacterial Transformation Lab were to observe standard bacterial growth under various conditions including the transformation of bacteria; to understand how the process of transformation occurs, as well as the biological results and consequences that come of transformation. In order to correctly perform this lab, plasmids, agar plates, Escherichia coli colonies, as well as incubation periods were used. The data that was collected was completely opposite than what the actual outcome should have been. Yes there was growth in the agar plates however there was not as much growth as predicted. Another problem that arose was the fact that the pGLO did not attach to any Escherichia coli DNA, so when under a UV light, none of the agar plates glowed, even the ones that were supposed to. After observing and recording the results that were collected, it can be concluded that due to human …show more content…
coli. This bacteria is a Gram-negative, facultative anaerobic, rod-shaped bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms. Bacterial transformation is the process by which bacterial cells take up naked DNA molecules. If the foreign DNA has an origin of replication recognized by the host cell DNA polymerases, the bacteria will replicate the foreign DNA along with their own DNA. The purpose of this technique is to introduce a foreign plasmid into the bacteria E. coli. A plasmid is a small, circular DNA molecule that contains important genetic information for the growth of bacteria. Some plasmids carry genes for antibiotic resistance or other traits that increase the cell’s ability to grow in a particular
Byanka Delgado
BIO 181 Lab Thursday 1:30-4:15
Arizona State University
Abstract The major objectives of the Bacterial Transformation Lab were to observe standard bacterial growth under various conditions including the transformation of bacteria; to understand how the process of transformation occurs, as well as the biological results and consequences that come of transformation. In order to correctly perform this lab, plasmids, agar plates, Escherichia coli colonies, as well as incubation periods were used. The data that was collected was completely opposite than what the actual outcome should have been. Yes there was growth in the agar plates however there was not as much growth as predicted. Another problem that arose was the fact that the pGLO did not attach to any Escherichia coli DNA, so when under a UV light, none of the agar plates glowed, even the ones that were supposed to. After observing and recording the results that were collected, it can be concluded that due to human …show more content…
coli. This bacteria is a Gram-negative, facultative anaerobic, rod-shaped bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms. Bacterial transformation is the process by which bacterial cells take up naked DNA molecules. If the foreign DNA has an origin of replication recognized by the host cell DNA polymerases, the bacteria will replicate the foreign DNA along with their own DNA. The purpose of this technique is to introduce a foreign plasmid into the bacteria E. coli. A plasmid is a small, circular DNA molecule that contains important genetic information for the growth of bacteria. Some plasmids carry genes for antibiotic resistance or other traits that increase the cell’s ability to grow in a particular