In this experiment, the effect of environmental conditions on enzyme functions was tested. The materials needed for the experiment was an amylase enzyme to lower the activation energy of the reactions, starch solutions of pH 3,4,5,6,7,and 8, these provided different concentrations to project various environmental conditions. In addition, Iodine solution, and a spectrophotometer were needed.
Each cuvette contained a specific pH concentration. Then the enzyme extract was added to each pH concentration for a reaction to take place. Once the reaction was completed in ten minutes, HCl was added, as an inhibitor, to each pH and enzyme containing cuvette. Afterwards, with parafilm at the top, the cuvettes were flipped over to pause the enzyme activity.
Each cuvette contained a specific pH concentration. Then the enzyme extract was added to each pH concentration for a reaction to take place. Once the reaction was completed in ten minutes, HCl was added, as an inhibitor, to each pH and enzyme containing cuvette. Afterwards, with parafilm at the top, the cuvettes were flipped over to pause the enzyme activity.