Introduction
When analyzing biological processes and development, Drosophila melanogaster is an ideal organism to utilize. Compared to other organisms, the functions of D. melanogaster are easier to manipulate due to its quick sedation time from carbon dioxide. These flies can be sedated in less than ten seconds with a carbon dioxide flow rate of 5L/min. D. melanogaster also produces offspring in a short amount of time (roughly 10 days at 25 degrees Celsius), which makes it a desirable species to use when results are needed in a short amount of time (Jennings 2011). These two characteristics allow a variety of experiments to be conducted on D. melanogaster. For example, different strains of DNA were injected into the embryos of D. melanogaster …show more content…
CPD has three different CP-like domains. The first and second domains are active, while the third is inactive. In D. melangaster, CPD undergoes splicing, produces six forms of mRNA, and then produces proteins (Holger et al. 2011). It seems that the timing for expression of CPD is different compared to wild-type flies because Gal4 is expressed and encourages the transgene expression for the upstream activating sequence (UAS) promotor. Because of this timing difference, certain CPD substrates may not be processed in the time it would take a wild-type fly to process them, which could explain the different characteristics between the flies (Sidyelyeva et al. …show more content…
This incorrect fold is what may give rise to the "sv" "r" ^"1" pointed wing mutation. The "sv" "r" ^"poi" variant has a nonsense mutation, which means that the protein does not form to its normal length. Because of this, the protein is not completely functional, which alters the function of the normally produced protein. Some of these proteins act as growth factors in D. melanogaster (Sidyelyeva et al. 2006). The alteration of growth-factor proteins in "sv" "r" ^"1" and "sv" "r" ^"poi" mutants could explain the changes in wing phenotype (Sidyelyeva et al.
When analyzing biological processes and development, Drosophila melanogaster is an ideal organism to utilize. Compared to other organisms, the functions of D. melanogaster are easier to manipulate due to its quick sedation time from carbon dioxide. These flies can be sedated in less than ten seconds with a carbon dioxide flow rate of 5L/min. D. melanogaster also produces offspring in a short amount of time (roughly 10 days at 25 degrees Celsius), which makes it a desirable species to use when results are needed in a short amount of time (Jennings 2011). These two characteristics allow a variety of experiments to be conducted on D. melanogaster. For example, different strains of DNA were injected into the embryos of D. melanogaster …show more content…
CPD has three different CP-like domains. The first and second domains are active, while the third is inactive. In D. melangaster, CPD undergoes splicing, produces six forms of mRNA, and then produces proteins (Holger et al. 2011). It seems that the timing for expression of CPD is different compared to wild-type flies because Gal4 is expressed and encourages the transgene expression for the upstream activating sequence (UAS) promotor. Because of this timing difference, certain CPD substrates may not be processed in the time it would take a wild-type fly to process them, which could explain the different characteristics between the flies (Sidyelyeva et al. …show more content…
This incorrect fold is what may give rise to the "sv" "r" ^"1" pointed wing mutation. The "sv" "r" ^"poi" variant has a nonsense mutation, which means that the protein does not form to its normal length. Because of this, the protein is not completely functional, which alters the function of the normally produced protein. Some of these proteins act as growth factors in D. melanogaster (Sidyelyeva et al. 2006). The alteration of growth-factor proteins in "sv" "r" ^"1" and "sv" "r" ^"poi" mutants could explain the changes in wing phenotype (Sidyelyeva et al.