Disinfectant Lab Report

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The aim of this experiment is to determine how best to remove bacteria from an environment. Disinfectants are formulations of active chemicals that sterilize inanimate surfaces or objects, whereas antiseptics deter bacterial growth on living tissue. These agents have two modes of anti-bacterial action; growth inhibition or lethal action.
Disease causing micro-organisms thrive wherever there are appropriate conditions to provide sustenance. There are four major types of pathogens; bacteria, fungi, viruses and protozoa. Determining the disinfectant with highest efficacy is important for aiding the prevention of the diseases, illnesses and infections caused by these pathogens.
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If synthetic disinfectants are applied on bacterium, they will be more effective because they comprise of a wider range of active ingredients specified to target microorganisms.
Eight filter paper discs were saturated into different disinfectants- four discs into synthetic and the other four into natural disinfectants. Using sterile forceps, the discs were placed into the corresponding labelled quadrants of two petri dishes which had been inoculated with the specific bacterium, Micrococcus luteus. The dishes were then cultured in an incubator over a period of four days.
The incubation period, paper disc size, agar nutrient type, bacterial culture and incubator temperature were kept constant. After changing the types of anti-bacterial substances used, the effectiveness of each agent was able to be measured through their inhibition zones.
If synthetic disinfectants are applied on the bacterium, they will be more effective because they comprise of a wider range of active ingredients that are specified to target

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