Daily Log:
Monday:
On Monday, Maggie was unable to come into the lab today as she was sick, so Cole continued to work on the research plan. He edited the methods section, adding paragraphs on RNA isolation and miRNA preparation for PCR. During our lab lunch meeting, Dr. Ericson and Cole discussed a study he found that looked at Bartonella in histiocytomas, skin tumors made of malignant dendritic cells. In this study, they used a confocal microscope and found Bartonella in histiocytomas of dogs infected with Bartonellosis; however, they were unable to conclude whether Bartonella caused histiocytomas. This study also ran PCR on the skin samples to detect Bartonella DNA. Even though this study looked at histiocytomas rather than melanoma tumors, it follows a similar procedure to the one we will be undergoing in our project.
Tuesday:
On Tuesday, …show more content…
Ericson to discuss our training. For the next two weeks, we will be staining feline gonads using the same process we will be undergoing when preparing the melanoma samples. We will then be image these gonads and learn how to use the confocal microscope in the imaging center. At the end of the day, we reviewed the staining protocol and decided what specific primary antibodies, secondary antibodies, and nuclear stains we will be using.
Thursday:
On Thursday, Maggie was unable to come into the lab, she continued her literature search.
Friday:
On Friday, we spent the morning with Jamie at the imaging center taking images of the Bartonella samples she prepared a few weeks. She taught us how to calibrate the microscope for specific antibodies and stains, and how to identify Bartonella and other cell types in tissue samples. In the afternoon, we began the process of preparing the feline gonads for staining by deparaffinizing the paraffin embedded cat gonads. We also labeled each of the wells with the antibodies and stains that will be
Monday:
On Monday, Maggie was unable to come into the lab today as she was sick, so Cole continued to work on the research plan. He edited the methods section, adding paragraphs on RNA isolation and miRNA preparation for PCR. During our lab lunch meeting, Dr. Ericson and Cole discussed a study he found that looked at Bartonella in histiocytomas, skin tumors made of malignant dendritic cells. In this study, they used a confocal microscope and found Bartonella in histiocytomas of dogs infected with Bartonellosis; however, they were unable to conclude whether Bartonella caused histiocytomas. This study also ran PCR on the skin samples to detect Bartonella DNA. Even though this study looked at histiocytomas rather than melanoma tumors, it follows a similar procedure to the one we will be undergoing in our project.
Tuesday:
On Tuesday, …show more content…
Ericson to discuss our training. For the next two weeks, we will be staining feline gonads using the same process we will be undergoing when preparing the melanoma samples. We will then be image these gonads and learn how to use the confocal microscope in the imaging center. At the end of the day, we reviewed the staining protocol and decided what specific primary antibodies, secondary antibodies, and nuclear stains we will be using.
Thursday:
On Thursday, Maggie was unable to come into the lab, she continued her literature search.
Friday:
On Friday, we spent the morning with Jamie at the imaging center taking images of the Bartonella samples she prepared a few weeks. She taught us how to calibrate the microscope for specific antibodies and stains, and how to identify Bartonella and other cell types in tissue samples. In the afternoon, we began the process of preparing the feline gonads for staining by deparaffinizing the paraffin embedded cat gonads. We also labeled each of the wells with the antibodies and stains that will be